Recombinant Human RCHY1 Protein, Myc/DDK-tagged

• Cat.No.: RCHY1-234H
• Product Overview: Recombinant protein of human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1 with a C-Myc/DDK tag was expressed in HEK293T.

Specification

• Description: The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 29.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Gene Information

• Gene Name: RCHY1 ring finger and CHY zinc finger domain containing 1 [ Homo sapiens (human) ]
• Official Symbol: RCHY1
• Synonyms: RCHY1; ring finger and CHY zinc finger domain containing 1; ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996; RING finger and CHY zinc finger domain-containing protein 1; CH-rich interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; RING finger protein 199; RING-type E3 ubiquitin transferase RCHY1; androgen-receptor N-terminal-interacting protein; p53-induced protein with a RING-H2 domain; ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase; zinc finger protein 363; zinc finger, CHY-type; EC 2.3.2.27
• Gene ID: 25898
• mRNA Refseq: NM_015436
• Protein Refseq: NP_056251
• MIM: 607680
• UniProt ID: Q96PM5

Reviews

11/25/2019

Experiments using RCHY1 were very reproducible and allowed me to evaluate the results more accurately.

07/18/2019

Due to stability of RCHY1 was still very good under different experimental conditions, indicating that it has a wide range of applications and adaptability.

04/10/2019

The band definition of RCHY1 in Western blot was very high, which improved the confidence of the experiment.

Q&As

Why RCHY1 attempts to provide supportive information as a marker in early node-negative oral squamous cell carcinoma?

RCHY1 acts as an independent target in early-stage node-negative oral squamous cell carcinoma and may provide patients with more accurate diagnostic and prognostic information.

What is the association between RCHY1 and ovarian, breast cancer, etc.?

The association between RCHY1 and ovarian and breast cancer is mainly manifested in its regulation of hormone receptor expression, migration and invasion.

Is RCHY1 an important driver?

RCHY1, as a Ubiquitin ligase, has been found as a driving factor in a variety of cancers such as liver, prostate, and kidney cancer.

How about the relationship between the expression of RCHY1 and prognosis?

RCHY1 is expressed in different types of cancer, and its high expression is associated with poor prognosis and high risk of metastasis.

Does the polymorphism of RCHY1 predict tumor recurrence?

For breast and ovarian cancer patients, the polymorphisms of RCHY1 are associated with the risk of tumor recurrence and can be used as a biomarker to predict recurrence.

What is the role of RCHY1 in apoptosis?

RCHY1 is involved in regulating apoptosis signaling pathways by mediating the single ubiquitination pathway and promoting apoptosis of tumor cells.