Recombinant Human REV1, His-tagged

• Cat.No.: REV1-175H
• Product Overview: REV1,51-256aa, Human, His tag, E.coli

Specification

• Description: REV1 contains a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found. Recombinant human REV1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 25.2 kDa (227aa)
• Protein length: 51-256aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGVAIYVNGY TDPSAEELRK LMMLHGGQYH VYYSRSKTTH IIATNLPNAK IKELKGEKVI RPEWIVESIK AGRLLSYIPY QLYTKQSSVQ KGLSFNPVCR PEDPLPGPSN IAKQLNNRVN HIVKKIETEN EVKVNGMNSW NEEDENNDFS FVDLEQTSPG RKQNGIPHPR GSTAIFNGHT PSSNGALKTQ DCLVPMVNSV ASRLSPA
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: REV1 REV1 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: REV1
• Synonyms: REV1; REV1 homolog (S. cerevisiae); REV1 (yeast homolog) like , REV1 like (yeast) , REV1L; DNA repair protein REV1; AIBP80; REV1- like; alpha integrin-binding protein 80; rev1-like terminal deoxycytidyl transferase; REV1L; FLJ21523; MGC26225; MGC163283
• Gene ID: 51455
• mRNA Refseq: NM_001037872
• Protein Refseq: NP_001032961
• MIM: 606134
• UniProt ID: Q9UBZ9
• Chromosome Location: 2q11.1-q11.2
• Pathway: DNA Damage Bypass, organism-specific biosystem; DNA Repair, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Translesion synthesis by DNA polymerases bypassing lesion on DNA template, organism-specific biosystem; Translesion synthesis by HREV1, organism-specific biosystem
• Function: DNA-directed DNA polymerase activity; damaged DNA binding; deoxycytidyl transferase activity; magnesium ion binding; protein binding; transferase activity

Reviews

05/16/2023

Good solubility and stability in solution.

08/26/2021

In special environments, it has good stability.

05/21/2020

I have already recommended this brand to my colleagues and friends, and they are all very enthusiastic about this product.

Q&As

Does REV1 recombinant protein have a role in promoting tumor growth?

The REV1 recombinant protein may have a growth-promoting effect in some cases, but may have an inhibitory effect in others. The specific mechanism needs to be further studied.

Is the expression level of REV1 recombinant protein affected by cellular stress?

The expression level of REV1 recombinant protein may be affected by cellular stress, such as DNA damage, but the specific mechanism needs to be further studied.

Is there a potential application for REV1 recombinant protein in drug development?

As a protein related to DNA damage repair, the enzymatic activity of REV1 recombinant protein may provide a potential target for drug development, but a lot of research is still needed to determine its application prospect.

Is the DNA repair pathway involved in REV1 recombinant protein related to the sensitivity of anticancer drugs?

Yes, there may be a correlation between the DNA repair pathway involved in the REV1 recombinant protein and the sensitivity of certain anticancer drugs, which warrants further investigation.

Does REV1 recombinant protein have an effect on variation in the human genome?

The misintroduction of REV1 recombinant protein during DNA damage repair may lead to genomic variation, but the specific mechanism needs to be further studied.

In which experimental models has REV1 recombinant protein been studied?

REV1 recombinant protein has been extensively studied in cell culture (in vitro) and animal models (in vivo).