Recombinant Human RPE65 Protein, DYKDDDDK-tagged

• Cat.No.: RPE65-02H
• Product Overview: Recombinant protein from the full-length sequence of homo sapiens RPE65, retinoid isomerohydrolase (RPE65) (NM_000329), with a DYKDDDDK tag was expressed in human cells.

Specification

• Description: This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.
• Source: Human cells
• Species: Human
• Tag: DYKDDDDK
• Molecular Mass: 60.8 kDa
• Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (<1 EU/μg)
• Purity: > 90% by SDS-PAGE gel and Coomassie Blue staining
• Applications: Antigens, Western, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies; For research & development use only!
• Storage Buffer: Purified protein formulated in a sterile solution of TBS buffer, pH7.4, without any preservatives

Gene Information

• Gene Name: RPE65 retinoid isomerohydrolase RPE65 [ Homo sapiens (human) ]
• Official Symbol: RPE65
• Synonyms: RPE65; retinoid isomerohydrolase RPE65; p63; BCO3; LCA2; RP20; rd12; mRPE65; sRPE65; retinoid isomerohydrolase; BCO family, member 3
  RBP-binding membrane protein; RPE65, retinoid isomerohydrolase; all-trans-retinyl-palmitate hydrolase; lutein isomerase; meso-zeaxanthin isomerase; retinal pigment epithelium specific protein 65; retinal pigment epithelium-specific 65 kDa protein; retinal pigment epithelium-specific protein 65kDa; retinitis pigmentosa 20 (autosomal recessive); retinol isomerase; EC 3.1.1.64; EC 5.3.3.22
• Gene ID: 6121
• mRNA Refseq: NM_000329
• Protein Refseq: NP_000320
• MIM: 180069
• UniProt ID: Q16518

Reviews

09/04/2022

High-quality protein sequencing, an essential tool in our lab.

12/29/2020

Critical expertise in protein engineering, a trusted research resource.

02/01/2018

Reliable protein crystallization services, consistently delivers exceptional results.

Q&As

What is the impact of gene therapy targeting RPE65 on inherited retinal dystrophies?

Gene therapy targeting RPE65 shows promise in treating inherited retinal dystrophies, restoring some visual functions.

What role does RPE65 play in the conversion of vitamin A derivatives in the retina?

RPE65 facilitates the conversion of vitamin A derivatives, essential for the regeneration of visual pigments.

What are the mechanisms by which RPE65 mutations affect visual acuity and night vision?

Mutations in RPE65 impact visual acuity and night vision, leading to difficulties in low-light conditions.

How do alterations in RPE65 expression or activity affect age-related macular degeneration?

Changes in RPE65 may contribute to the progression of age-related macular degeneration, affecting central vision.

How does RPE65 influence the regeneration of photoreceptors and visual pigment?

RPE65 is involved in the regeneration of photoreceptors and visual pigments, key for maintaining healthy vision.

How does RPE65 (Retinal Pigment Epithelium-Specific 65kDa Protein) function in the visual cycle and retinal health?

RPE65 is crucial in the visual cycle, converting vitamin A derivatives necessary for retinal function and vision.

How does RPE65 deficiency contribute to retinal diseases like Leber congenital amaurosis and retinitis pigmentosa?

RPE65 deficiency leads to retinal diseases by disrupting the visual cycle, affecting vision from an early age.