Recombinant Mouse Rpe65 Protein, Myc/DDK-tagged

• Cat.No.: Rpe65-5581M
• Product Overview: Purified recombinant protein of mouse full-length retinal pigment epithelium 65 (Rpe65), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore. Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 61.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rpe65 retinal pigment epithelium 65 [ Mus musculus (house mouse) ]
• Official Symbol: Rpe65
• Synonyms: RPE65; retinal pigment epithelium 65; retinoid isomerohydrolase; retinol isomerase; modifier of retinal degeneration 1; retinal pigment epithelium, 65 kDa; all-trans-retinyl-palmitate hydrolase; retinal pigment epithelium-specific 65 kDa protein; LCA2; RP20; rd12; 65kDa; Mord1; A930029L06Rik
• Gene ID: 19892
• mRNA Refseq: NM_029987
• Protein Refseq: NP_084263
• UniProt ID: Q91ZQ5

Reviews

09/04/2022

High-quality protein sequencing, an essential tool in our lab.

12/29/2020

Critical expertise in protein engineering, a trusted research resource.

02/01/2018

Reliable protein crystallization services, consistently delivers exceptional results.

Q&As

What is the impact of gene therapy targeting RPE65 on inherited retinal dystrophies?

Gene therapy targeting RPE65 shows promise in treating inherited retinal dystrophies, restoring some visual functions.

What role does RPE65 play in the conversion of vitamin A derivatives in the retina?

RPE65 facilitates the conversion of vitamin A derivatives, essential for the regeneration of visual pigments.

What are the mechanisms by which RPE65 mutations affect visual acuity and night vision?

Mutations in RPE65 impact visual acuity and night vision, leading to difficulties in low-light conditions.

How do alterations in RPE65 expression or activity affect age-related macular degeneration?

Changes in RPE65 may contribute to the progression of age-related macular degeneration, affecting central vision.

How does RPE65 influence the regeneration of photoreceptors and visual pigment?

RPE65 is involved in the regeneration of photoreceptors and visual pigments, key for maintaining healthy vision.

How does RPE65 (Retinal Pigment Epithelium-Specific 65kDa Protein) function in the visual cycle and retinal health?

RPE65 is crucial in the visual cycle, converting vitamin A derivatives necessary for retinal function and vision.

How does RPE65 deficiency contribute to retinal diseases like Leber congenital amaurosis and retinitis pigmentosa?

RPE65 deficiency leads to retinal diseases by disrupting the visual cycle, affecting vision from an early age.