Recombinant Human RPE65 protein, His-tagged

• Cat.No.: RPE65-2372H
• Product Overview: Recombinant Human RPE65 protein(Ser2~Ser533), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 65kDa(Analysis of differences refer to the manual)
• Protein length: Ser2~Ser533
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: RPE65 retinal pigment epithelium-specific protein 65kDa [ Homo sapiens ]
• Official Symbol: RPE65
• Synonyms: RPE65; retinal pigment epithelium-specific protein 65kDa; retinal pigment epithelium specific protein (65kD) , RP20; retinoid isomerohydrolase; all trans retinyl palmitate hydrolase; LCA2; rd12; retinol isomerase; p63; RBP-binding membrane protein; all-trans-retinyl-palmitate hydrolase; retinitis pigmentosa 20 (autosomal recessive); retinal pigment epithelium-specific 65 kDa protein; RP20; mRPE65; sRPE65
• Gene ID: 6121
• mRNA Refseq: NM_000329.2
• Protein Refseq: NP_000320.1
• MIM: 180069
• UniProt ID: Q16518

Reviews

09/04/2022

High-quality protein sequencing, an essential tool in our lab.

12/29/2020

Critical expertise in protein engineering, a trusted research resource.

02/01/2018

Reliable protein crystallization services, consistently delivers exceptional results.

Q&As

What is the impact of gene therapy targeting RPE65 on inherited retinal dystrophies?

Gene therapy targeting RPE65 shows promise in treating inherited retinal dystrophies, restoring some visual functions.

What role does RPE65 play in the conversion of vitamin A derivatives in the retina?

RPE65 facilitates the conversion of vitamin A derivatives, essential for the regeneration of visual pigments.

What are the mechanisms by which RPE65 mutations affect visual acuity and night vision?

Mutations in RPE65 impact visual acuity and night vision, leading to difficulties in low-light conditions.

How do alterations in RPE65 expression or activity affect age-related macular degeneration?

Changes in RPE65 may contribute to the progression of age-related macular degeneration, affecting central vision.

How does RPE65 influence the regeneration of photoreceptors and visual pigment?

RPE65 is involved in the regeneration of photoreceptors and visual pigments, key for maintaining healthy vision.

How does RPE65 (Retinal Pigment Epithelium-Specific 65kDa Protein) function in the visual cycle and retinal health?

RPE65 is crucial in the visual cycle, converting vitamin A derivatives necessary for retinal function and vision.

How does RPE65 deficiency contribute to retinal diseases like Leber congenital amaurosis and retinitis pigmentosa?

RPE65 deficiency leads to retinal diseases by disrupting the visual cycle, affecting vision from an early age.