Recombinant Human RXRG 293 Cell Lysate

• Cat.No.: RXRG-2097HCL

Specification

• Description: Antigen standard for retinoid X receptor, gamma (RXRG), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RXRG retinoid X receptor, gamma [ Homo sapiens ]
• Official Symbol: RXRG
• Synonyms: RXRG; retinoid X receptor, gamma; retinoic acid receptor RXR-gamma; NR2B3; nuclear receptor subfamily 2 group B member 3; RXRC
• Gene ID: 6258
• mRNA Refseq: NM_001256570
• Protein Refseq: NP_001243499
• MIM: 180247
• UniProt ID: P48443
• Chromosome Location: 1q22-q23
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Non-small cell lung cancer, organism-specific biosystem; Non-small cell lung cancer, conserved biosystem
• Function: 9-cis retinoic acid receptor activity; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; metal ion binding; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid binding; steroid hormone receptor activity; zinc ion binding

Reviews

05/07/2018

Aids in groundbreaking discoveries, highly recommended.

12/29/2017

Outstanding protein analysis, a research asset.

08/01/2017

Enables data-driven decisions in our research.

Q&As

How do variations in the RXRG gene affect its function and contribute to disease?

Genetic mutations in RXRG can disrupt its regulatory roles, leading to various developmental and metabolic disorders.

What is the significance of RXRG in the regulation of metabolic pathways and homeostasis?

RXRG is key in regulating lipid and glucose metabolism, playing a role in metabolic homeostasis.

What role does RXRG play in developmental processes and organogenesis?

RXRG is essential in embryonic development and organ formation, guiding cell fate decisions.

What is the primary function of RXRG in cellular signaling and gene regulation?

RXRG functions as a nuclear receptor, regulating gene expression in response to ligand binding.

What potential does RXRG have as a therapeutic target in metabolic and degenerative diseases?

Targeting RXRG in therapy offers potential in treating metabolic syndromes and degenerative diseases.

How does RXRG interact with retinoids and what is its role in vitamin A metabolism?

It interacts with retinoids to modulate vitamin A metabolism, influencing cellular differentiation and proliferation.

How does RXRG contribute to the immune system's function and response?

It influences immune function by affecting the development and differentiation of immune cells.