Recombinant Human SLC40A1 protein, His & GST-tagged

• Cat.No.: SLC40A1-7816H
• Product Overview: Recombinant Human SLC40A1 aa. (Trp127~Val321 linked with LEHHHHHH) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a cell membrane protein that may be involved in iron export from duodenal epithelial cells. Defects in this gene are a cause of hemochromatosis type 4 (HFE4).
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: 52kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Trp127~Val321 linked with LEHHHHHH
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >80%
• Characteristic: The isoelectric point is 6.0.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: SLC40A1 solute carrier family 40 member 1 [ Homo sapiens (human) ]
• Official Symbol: SLC40A1
• Synonyms: Dtymk; deoxythymidylate kinase; thymidylate kinase
• Gene ID: 30061
• mRNA Refseq: NM_014585.5
• Protein Refseq: NP_055400.1
• UniProt ID: Q9NP59