Recombinant Mouse Adam10, His-tagged

• Cat.No.: Adam10-1927M
• Product Overview: Recombinant Mouse Adam10 (Thr215-Glu673), fused with a C terminal 10 His tag, was expressed in Sf 21 Cells.

Specification

• Cat. No.: Adam10-1927M
• Description: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. Sheddase, a generic name for the ADAM metallopeptidase, functions primarily to cleave membrane proteins at the cellular surface. Once cleaved, the sheddases release soluble ectodomains with an altered loca-tion and function.
• Source: Sf 21 Cells
• Molecular Mass: The predicted molecular mass of this protein is 52 KDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
• Purity: >90%, by SDSPAGE
• Bio-Activity: Measured by its ability to cleave a fluorogenic peptide substrate McaPLAQAVDpaRSSSR-NH2. The specific activity is >5 pmol/min/μg
• Form: Lyophilized from a 0.2 μm filtered solution in MES, NaCl, CaCl2, ZnCl2, and Brij35.
• EndotoxinLevel: <1.0 EU per 1 μg of the protein by the LAL method.
• Reconstitution: Reconstitute at 100 μg/mL in sterile 25 mM Tris, pH 7.5.
• Storage: Store at -200C ~ -700C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: Adam10

Gene Information

• Gene Name: Adam10 a disintegrin and metallopeptidase domain 10 [ Mus musculus ]
• Synonyms: Adam10; a disintegrin and metallopeptidase domain 10; kuz; MADM; kuzbanian; 1700031C13Rik; Adam10
• Gene ID: 11487
• mRNA Refseq: NM_007399
• Protein Refseq: NP_031425
• UniProt ID: O35598
• Chromosome Location: 9 D; 9 41.0 cM
• Pathway: Alzheimer"s disease
• Function: SH2 domain binding; SH3 domain binding; integrin binding; metal ion binding; metalloendopeptidase activity; peptidase activity; protein homodimerization activity; protein kinase binding; zinc ion binding; metalloendopeptidase activity

Reviews

09/16/2021

During the experiment, the technician helped me solve the problems in a timely and accurate manner.

04/03/2021

The ADAM10 proteins purchased from your company is suitable for WB.

09/08/2020

Received high quality protein products and obtained satisfactory experimental results.

Q&As

What proteins does ADAM10 cleave?

ADAM10 protein can cleave TNF-α and E-cadherin, etc.

Does adding GST tag have any effect on protein?

The addition of the GST tag helps protect the recombinant protein from extracellular protease degradation and improves its stability.

How to distinguish between ADAM10 precursors and active bodies?

The protein size of ADAM10 precursor is larger than that of ADAM10 active.

In addition to cardiovascular tumor-related research, ADAM10 is currently used in the research of which diseases?

ADAD1 is currently also used in the research of Alzheimer's disease and diabetic retinopathy, etc.