Active Recombinant Mouse ADAM15 Protein, His-tagged

• Cat.No.: ADAM15-948M
• Product Overview: Recombinant mouse ADAM15 (O88839-1) extracellular domain (Met 1-Thr 697) was expressed, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Predicted N Terminal: Arg 18 & Asp 208
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its ability to bind human PTK6 in a functional ELISA. 2. Measured by its ability to bind human SRC in a functional ELISA. 3. Measured by its ability to bind mouse SRC in a functional ELISA. 4. Measured by its ability to bind human LYN in a
• Molecular Mass: The secreted recombinant mouse ADAM15 (pro form) comprises 691 amino acids and has a calculated molecular mass of 74.6 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Adam15 a disintegrin and metallopeptidase domain 15 (metargidin) [ Mus musculus ]
• Official Symbol: ADAM15
• Synonyms: ADAM15; a disintegrin and metallopeptidase domain 15 (metargidin); disintegrin and metalloproteinase domain-containing protein 15; AD56; MDC-15; ADAM 15; disintegrin and metalloprotease 15; metalloprotease RGD disintegrin protein; a disintegrin and metall
• Gene ID: 11490
• mRNA Refseq: NM_001037722
• Protein Refseq: NP_001032811
• Function: SH3 domain binding; hydrolase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; protein binding; zinc ion binding

Reviews

09/04/2021

The purchased protein is suitable for WB experiments and I am satisfied with the results.

05/17/2020

The technical expert communicated with me in time to understand the progress of my experiment and gave me suggestions for improvement.

Q&As

In addition to breast cancer research, what other diseases is ADAM15 currently used to study?

ADAM15 used in studies of liver and bladder cancers.

I want to buy ADAM15-related proteins, do you provide other proteins?

We offer MMP9 and MMP2 proteins in addition to ADAM15 proteins.

Does ADAM15 affect H460 expression?

ADAM15 affects the proliferation of H460 cells by regulating PI3K-AKT and MAPK-ERK signaling pathways.

Does adding GST tag have any effect on protein?

Addition of GST tags can accelerate the solubilization of certain insoluble proteins.