Recombinant Mouse ADAMTS12, His-tagged

• Cat.No.: ADAMTS12-3006M
• Product Overview: A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12)

Specification

• Description: This gene encodes a large abundant protein of striated muscle. The product of this gene is divided into two regions, a N-terminal I-band and a C-terminal A-band. The I-band, which is the elastic part of the molecule, contains two regions of tandem immunoglobulin domains on either side of a PEVK region that is rich in proline, glutamate, valine and lysine. The A-band, which is thought to act as a protein-ruler, contains a mixture of immunoglobulin and fibronectin repeats, and possesses kinase activity. An N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere, respectively, so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Alternative splicing of this gene results in multiple transcript variants. Considerable variability exists in the I-band, the M-line and the Z-disc regions of titin. Variability in the I-band region contributes to the differences in elasticity of different titin isoforms and, therefore, to the differences in elasticity of different muscle types. Mutations in this gene are associated with familial hypertrophic cardiomyopathy 9, and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma. [provided by RefSeq, Feb 2012]
• Source: E. Coli
• Species: Mouse
• Tag: His
• Form: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 21.0kDa
• Protein length: 34350
• Endotoxin: <1.0eu per 1ug (determined by the lal</1.0eu
• Purity: >95%
• Applications: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Notes: Small volumes of ADAMTS12 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: Adamts12 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12 [ Mus musculus ]
• Official Symbol: ADAMTS12
• Synonyms: ADAMTS12; a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12; A disintegrin and metalloproteinase with thrombospondin motifs 12; ADAM-TS12; ADAM-TS 12; metalloprotease disintegrin 12; a disintegrin-like and metalloprotease with thrombospondin type 1 motif, 12; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 12; AI605170; ADAMTS-12
• Gene ID: 239337
• mRNA Refseq: NM_175501
• Protein Refseq: NP_780710
• Function: hydrolase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding

Reviews

01/16/2023

I got this product at a discounted price, with some discounted prices. It also works well for ELISA.

05/05/2021

Fast shipping, quality product, right price, worth buying!

03/01/2021

Customer service patiently communicated with me and helped me to determine the experimental matters.

02/24/2021

The purchased ADAMTS12 protein is suitable for WB experiments and I am satisfied with the results.

Q&As

How long will it take to ship?

We will ship as soon as possible after your purchase.

Is ADAMTS12 involved in the mitogen activated protein kinases signaling pathway?

ADAMTS12 blocked the mitogen activated protein kinase signaling pathway and prevented the tumorigenic effect of hepatocyte growth factor.

What diseases is ADAMTS12 currently used in research?

ADAMTS12 is more frequently studied in pancreatic and liver cancers.

Can the labeling of the full-length protein be changed?

We can customize the production products according to your needs.

Is ADAMTS12 expressed in normal tissues?

Not expressed, ADAMTS12 protein is only widely expressed in malignant digestive system tumor tissues.

Does ADAMTS12 affect epithelial-mesenchymal transition(EMT)?

Yes, reducing the expression of ADAMTS12 interferes with the EMT process.

What are the biological functions of ADAMTS12?

ADAMTS12 has the capabilities of tissue remodeling and cell migration and may work during normal inflammatory response.

I want to buy ADAMTS12 for western blotting, can you recommend it?

You can contact our experts directly, and they will recommend suitable products for you according to your research purpose.