Recombinant Mouse Il15 protein, Fc-tagged

• Cat.No.: Il15-647M
• Product Overview: Recombinant Mouse Il15 (Asn49-Ser162) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Interleukin 15 is also known as IL15, IL-15, and is a cytokine with structural similarity to IL-2. Like IL-2, IL-15 binds to and signals through the IL-2/IL-15 beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). This cytokine induces cell proliferation of natural killer cells; cells of the innate immune system whose principal role is to kill virally infected cells. Interleukin 15 (IL-15) regulates T and natural killer (NK) cell activation and proliferation. Survival signals that maintain memory T cells in the absence of antigen are provided by IL-15. This cytokine is also implicated in NK cell development. In rodent lymphocytes, IL-15 prevents apoptosis by inducing an apoptosis inhibitor, BCL2L1/BCL-x(L). IL-15 has been shown to enhance the anti-tumor immunity of CD8+ T cells in pre-clinical models. A phase I clinical trial to evaluate the safety, dosing, and anti-tumor efficacy of IL-15 in patients with metastatic melanoma and renal cell carcinoma (kidney cancer) has begun to enroll patients at the National Institutes of Health.
• Source: HEK293
• Species: Mouse
• Tag: human IgG1 Fc
• Predicted N Terminal: Asn49
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 39.7 kDa. The protein migrates as 49 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Asn49-Ser162
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Il15
• Official Symbol: Il15
• Synonyms: IL15; interleukin 15; interleukin-15; IL-15; AI503618
• Gene ID: 16168
• mRNA Refseq: NM_001254747
• Protein Refseq: NP_001241676
• UniProt ID: P48346

Reviews

04/19/2018

Mapping protein- interactions in DNA transcription for gene regulation.

02/20/2018

Suitable for cytotoxicity assays.

10/22/2017

Effective in NK cell activation.

Q&As

What techniques differentiate IL15 and IL2 receptor binding kinetics?

Techniques discern IL15 and IL2 receptor binding kinetics.

What experimental methods study IL15's involvement in autoimmune diseases?

Methods explore IL15's role in autoimmune disease mechanisms.

What are the applications of IL15 knockout models in deciphering its functions?

IL15 knockout models provide insights into its diverse functions.

How do you analyze IL15's impact on memory T cell differentiation?

IL15's influence on memory T cell differentiation investigated.

How does immunofluorescence microscopy visualize IL15 localization in cells?

Immunofluorescence microscopy visualizes IL15 subcellular localization.

What approaches validate IL15's role in inflammatory responses?

Approaches validate IL15's involvement in inflammatory responses.

Could you explain the role of IL15 in NK cell cytotoxicity assays?

IL15's role in NK cell cytotoxicity analyzed using assays.

Can you detail the use of ELISA in quantifying IL15 secretion?

ELISA measures IL15 secretion accurately.

How is IL15's effect on regulatory T cells quantified using qPCR?

qPCR quantifies IL15's impact on regulatory T cells.

How does flow cytometry assess IL15-induced T-cell proliferation?

Flow cytometry quantifies IL15-driven T-cell proliferation.