Recombinant Mouse Rpa1 Protein, Myc/DDK-tagged

• Cat.No.: Rpa1-5578M
• Product Overview: Purified recombinant protein of mouse full-length replication protein A1 (Rpa1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 69 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rpa1 replication protein A1 [ Mus musculus (house mouse) ]
• Official Symbol: Rpa1
• Synonyms: RPA1; replication protein A1; replication protein A 70 kDa DNA-binding subunit; RP-A p70; RF-A protein 1; replication factor A protein 1; Rpa; RF-A; RP-A; 70kDa; AA589576; AW557552; 5031405K23Rik
• Gene ID: 68275
• mRNA Refseq: NM_026653
• Protein Refseq: NP_080929
• UniProt ID: Q8VEE4

Reviews

04/20/2021

The background of RPA1 in Western blot was very clean and showed good antibody specificity.

02/09/2019

Beacuse reproducibility of RPA1 was very good and consistent results were obtained in each experiment, which gave me more confidence in the experimental results.

01/27/2019

RPA1 have a wide range of applications and can be used in various biological experiments.

Q&As

What is the importance of RPA1 protein in DNA recombination?

RPA1 protein is involved in important steps such as pairing, redox, and fusion in DNA recombination, and is necessary to effectively maintain genome integrity and stability.

What is the role of RPA1 protein in embryonic development?

RPA1 protein participates in DNA damage repair, growth and development in embryonic development, and has a role in maintaining the genome in embryos.

Can RPA1 protein be used to monitor or detect oxidative damage to DNA?

Since RPA1 protein plays an important role in DNA oxidative damage repair, it can be used as a marker of the degree of DNA oxidative damage to monitor and detect the risk of related diseases.

Is the RPA1 protein involved in DNA methylation modifications?

Although RPA1 protein is not a factor directly involved in DNA methylation modification, it may be involved in DNA methylation by participating in processes such as DNA damage repair, chromatin structure, and regulation of gene expression.

What role does RPA1 play in diabetes?

Studies have shown that RPA1 protein is involved in processes such as insulin secretion and regulation of β cell function in diabetes, and may become a new target for therapeutic strategies.

Is RPA1 protein involved in the immune system?

The RPA1 protein is involved in DNA damage repair and cell cycle in the immune system, and studies have found that it may be involved in processes such as immune response and pattern recognition.