Recombinant Rat Rpa1 Protein, His-tagged

• Cat.No.: Rpa1-2029R
• Product Overview: Recombinant Rat Rpa1 Protein (Ser486-Ile680) with N-His tag was expressed in E. coli.

Specification

• Description: Enables single-stranded DNA binding activity. Predicted to be involved in several processes, including DNA metabolic process; DNA unwinding involved in DNA replication; and protein localization to chromosome. Predicted to act upstream of or within several processes, including hemopoiesis; homeostasis of number of cells within a tissue; and in utero embryonic development. Part of DNA replication factor A complex. Orthologous to human RPA1 (replication protein A1).
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.3 kDa
  Accurate Molecular Mass: 26 kDa
• Protein length: Ser486-Ile680
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Rpa1 replication protein A1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Rpa1
• Synonyms: RPA1; replication protein A1; replication protein A 70 kDa DNA-binding subunit; Cb1-727
• Gene ID: 287524
• mRNA Refseq: NM_001047843
• Protein Refseq: NP_001041308
• UniProt ID: Q7TP21

Reviews

04/20/2021

The background of RPA1 in Western blot was very clean and showed good antibody specificity.

02/09/2019

Beacuse reproducibility of RPA1 was very good and consistent results were obtained in each experiment, which gave me more confidence in the experimental results.

01/27/2019

RPA1 have a wide range of applications and can be used in various biological experiments.

Q&As

What is the importance of RPA1 protein in DNA recombination?

RPA1 protein is involved in important steps such as pairing, redox, and fusion in DNA recombination, and is necessary to effectively maintain genome integrity and stability.

What is the role of RPA1 protein in embryonic development?

RPA1 protein participates in DNA damage repair, growth and development in embryonic development, and has a role in maintaining the genome in embryos.

Can RPA1 protein be used to monitor or detect oxidative damage to DNA?

Since RPA1 protein plays an important role in DNA oxidative damage repair, it can be used as a marker of the degree of DNA oxidative damage to monitor and detect the risk of related diseases.

Is the RPA1 protein involved in DNA methylation modifications?

Although RPA1 protein is not a factor directly involved in DNA methylation modification, it may be involved in DNA methylation by participating in processes such as DNA damage repair, chromatin structure, and regulation of gene expression.

What role does RPA1 play in diabetes?

Studies have shown that RPA1 protein is involved in processes such as insulin secretion and regulation of β cell function in diabetes, and may become a new target for therapeutic strategies.

Is RPA1 protein involved in the immune system?

The RPA1 protein is involved in DNA damage repair and cell cycle in the immune system, and studies have found that it may be involved in processes such as immune response and pattern recognition.