Recombinant S. pyogenes Cas9 Protein, His-tagged
| Cat.No. : | Cas9-06S |
| Product Overview : | Recombinant full length Cas9-NLS from S. pyogenes was expressed in E. coli using a C-terminal His tag. Cas9-NLS contains a nuclear localization sequence for optimal activity. |
- Specification
- Gene Information
- Related Products
| Description : | In bacteria and archaea CRISPR- Cas systems provide sequence based adaptive immunity against mobile genetic elements such as phages and plasmids. Cas9 is a multidomain nuclease from the bacterium S. pyogenes characteristic of type II CRISPR- Cas systems. Using single guide RNA (sgRNA), Cas9 is targeted to specific genomic loci where it generates a blunt ended double strand beak. Cas9 can be used for genetic engineering experiments such as genome editing and generating transgenic models. |
| Source : | E. coli |
| Species : | S. pyogenes |
| Tag : | His |
| Molecular Mass : | Observed MW: ~160 kDa Calculated MW: ~161 kDa |
| Purity : | >70% |
| Stability : | One year at -70 centigrade from deta of shipment. |
| Storage : | Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Always include RNase inhibitor at manufacturer recommended concentrations in Cas enzyme reactions. |
| Concentration : | 0.1 μg/μL 6.26 pmol/μg |
| Storage Buffer : | Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol. |
| Shipping : | On dry ice. |
| Official Symbol : | Cas9 |
| Synonyms : | Csn1; Csx12; Spy_1046; SpyCas9 |
| Protein Refseq : | AAK33936 |
| UniProt ID : | Q99ZW2 |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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