Recombinant Zebrafish ACAP3B

• Cat.No.: ACAP3B-5338Z
• Product Overview: Recombinant Zebrafish ACAP3B full length or partial length protein was expressed.

Specification

• Source: Mammalian Cells
• Species: Zebrafish
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 oC for short term. For long term storage, store it at -20 oC～-80 oC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: acap3b ArfGAP with coiled-coil, ankyrin repeat and PH domains 3b [ Danio rerio (zebrafish) ]
• Official Symbol: ACAP3B
• Gene ID: 100009646
• mRNA Refseq: NM_001083015
• Protein Refseq: NP_001076484
• UniProt ID: A2RUY3

Reviews

06/18/2021

The scientists were able to customize the recombinant protein to my exact specifications, and the product worked perfectly for my project.

10/12/2020

Their Recombinant Zebrafish ACAP3B arrived quickly and was exactly what I needed for my project."

09/18/2020

Their technical support team was able to create a product that met my exact specifications.

Q&As

I am going to purchase ACAP3B proteins, can I get a discount on the price?

Discounts are available for first-time purchases and for holidays. For specific promotions, please contact our customer service staff. Thank you for your inquiry and we look forward to your order.

Does His tag affect ACAP3B protein activity?

Protein tags may or may not affect protein activity. For some applications, His tags may not affect protein activity and therefore need not be removed. However, protein tags are useful for affinity purification or for a variety of other purposes. If you are concerned about tags interfering with protein activity, Creative BioMart can also offer tag-free recombinant ACAP3B protein customization services.

I would like to buy ACAP3B recombinant protein, is there any discount?

We offer coupons for each new customer and run promotions on important holidays. Follow our website for the latest promotions.

What is the structural basis of ACAP3B's interaction with ARF6?

The interaction between ACAP3B and ARF6 is mediated by the PH domain of ACAP3B, which binds to the N-terminal helix of ARF6. This interaction results in the recruitment of ACAP3B to the plasma membrane and its subsequent activation as a GAP for ARF6. The coiled-coil domain of ACAP3B is also involved in its interaction with ARF6 and plays a role in regulating its activity.

What is the expression pattern of ACAP3B in different tissues?

ACAP3B is widely expressed in different tissues, with higher expression levels detected in brain, heart, and kidney tissues. It is also expressed in various cancer cell lines, suggesting its potential role in cancer development and progression.

What is the role of ACAP3B in endocytosis?

ACAP3B has been shown to play a role in endocytosis by regulating the internalization of cell surface receptors. It interacts with the clathrin adaptor protein AP-2 and is involved in the formation of clathrin-coated pits, which are responsible for the internalization of various receptors from the cell surface.

How does ACAP3B contribute to cytokinesis?

ACAP3B is involved in cytokinesis, the final stage of cell division, by regulating the trafficking of membrane vesicles to the cleavage furrow. It interacts with the centralspindlin complex, which is required for the assembly of the contractile ring that drives cytokinesis.

What is the function of ACAP3B protein in membrane trafficking?

ACAP3B protein is involved in membrane trafficking and vesicle formation through its interaction with the small GTPase ARF6. It acts as a GTPase-activating protein (GAP) for ARF6, which regulates membrane trafficking and fusion events in the cell.

What applications can the recombinant ACAP3B protein be used for?

Recombinant ACAP3B protein can be used for various applications, including biochemical assays, protein-protein interaction studies, and antibody production. Its role in membrane trafficking and vesicle formation makes it particularly useful for studying cellular processes related to endocytosis and cytokinesis.

Are there any precautions or considerations to keep in mind when working with recombinant ACAP3B protein?

When working with any recombinant protein, it is important to follow proper handling and safety protocols to prevent contamination and ensure safety. It is also important to consider the protein's stability and activity under the specific experimental conditions being used. Consult our instructions or seek advice from a technical support representative for guidance on handling and using recombinant ACAP3B protein.