Recombinant Rat Il33 protein, His-tagged

• Cat.No.: Il33-50R
• Product Overview: Recombinant rat Il33, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Il33 also known as Interleukin-33. Il33 induces production of large amounts of IL5 and IL13 by group 2 innate lymphoid cells (ILC2s), for initiation of allergic inflammation shortly after exposure to allergens or infection with parasites or viruses. Il33 appears to function as an alarmin (alarm signal) rapidly released from producing cells upon cellular damage or cellular stress.
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline (pH7.4) containing 10% glycerol
• Molecular Mass: 19.8kDa (179aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSSIQGTSL LTESCALSTY NDQSVSFVLE NGCYVINVED CGKNQEKDKV LLRYYESSFP AQSGDGVDGK KLMVNMSPIK DTDIWLNAND KDYSVELQKG DVSPPDQAFF VLHKKSSDFV SFECKNLPGT YIGVKDNQLA LVEENDESCN NIMFKLSKM
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: Il33 interleukin 33 [ Rattus norvegicus ]
• Official Symbol: Il33
• Synonyms: IL33; interleukin 33; interleukin-33; IL-33; RGD1311155
• Gene ID: 361749
• mRNA Refseq: NM_001014166
• Protein Refseq: NP_001014188
• Pathway: Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Influenza A, organism-specific biosystem; Influenza A, conserved biosystem
• Function: cytokine activity; cytokine activity

Reviews

10/16/2021

Good solubility and is easy to operate and purify.

05/03/2021

Effectively mimics the function of the target protein in vitro.

03/18/2020

IL33 has a wide range of applications in the field of scientific research and is suitable for a variety of experimental design and research purposes.

Q&As

Which immune cells can recombinant IL33 be used to detect?

It can be used to detect immune cells such as macrophages and dendritic cells.

In what research fields can recombinant IL33 be applied?

This protein applied in immunology, drug research, life science and other fields.

What is the action mechanism of IL33?

Regulates the activity of immune cells by binding to receptors to stimulate the production of inflammatory factors such as interferon and TNF-α.

Besides treating asthma and hay fever, what other diseases can be treated with IL33?

IL33 can also be used to treat a variety of inflammatory diseases, such as inflammatory bowel disease, psoriasis and so on.

How to prepare IL33?

The preparation of IL33 requires first cloning the IL33 gene into the expression vector, and then the expression vector is expressed and purified in the cell culture medium.

How long does it take to treat asthma with IL33?

Treating asthma with recombinant IL33 takes several weeks to several months to be effective, depending on the severity of the condition and the individual patient.