Active Recombinant Human Interleukin 1, Beta
Cat.No. : | IL1B-461H |
Product Overview : | Interleukin-1beta (IL-1μ) is a proinflammatory cytokine produced in response to inflammatory agents by a variety of cells, including monocytes, macrophages, and dendric cells (DCs). IL-1μ and IL-1μ are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1μ is cell associated and IL-1μ is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Recombinant human IL-1μ is a non-glycosylated single chain 17,376 Da protein containing 153 amino acid residues. |
- Specification
- Gene Information
- Related Products
Cat. No. : | IL1B-461H |
Description : | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. |
Species : | Human |
Source : | E.coli |
Form : | Lyopohilized from 10 mM NaP, pH 7.5. |
Purity : | Purity Greater than 97% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
Bio-activity : | Human IL-1μ is fully biologically active when compared to a standard. The ED 50 , as determined by the dose-dependent proliferation of mouse D10S cells, is 1 pg/ml. |
Physical Appearance : | Sterile Filtered white lyophilized (freeze-dried) powder. |
Solubility : | It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions. |
Purity Greater than 97% as determined by : | Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
Protein Content determined by : | UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
Endotoxin Level : | Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
Amino acid sequence : | APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS |
Storage : | Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
Gene Name : | IL1B interleukin 1, beta [ Homo sapiens ] |
Official Symbol : | IL1B |
Synonyms : | IL1B; interleukin-1 beta; IL-1; IL1F2; IL1-BETA; IL-1 beta; catabolin; OTTHUMP00000162031; preinterleukin 1 beta; pro-interleukin-1-beta |
Gene ID : | 3553 |
mRNA Refseq : | NM_000576 |
Protein Refseq : | NP_000567 |
MIM : | 147720 |
UniProt ID : | P01584 |
Chromosome Location : | 2q14 |
Pathway : | African trypanosomiasis; Alzheimer"s disease; Amoebiasis; Apoptosis; Cellular roles of Anthrax toxin; Chagas disease (American trypanosomiasis); Cytokine Signaling in Immune system; Cytokine-cytokine receptor interaction; Cytokines and Inflammatory Response; Cytosolic DNA-sensing pathway; Graft-versus-host disease; Hematopoietic cell lineage; IFN-gamma pathway; IL12-mediated signaling events; Immune System; Interleukin-1 signaling |
Function : | interleukin-1 receptor binding; protein binding; signal transducer activity; cytokine activity; growth factor activity |
Products Types
◆ Recombinant Protein | ||
IL1B-250I | Active Recombinant Human IL1B Protein | +Inquiry |
IL1B-40H | Recombinant Human IL1B Protein | +Inquiry |
Il1b-249I | Active Recombinant Mouse Il1b Protein | +Inquiry |
IL1B-0849H | Recombinant Human IL1B Protein (A117-S269), Tag Free | +Inquiry |
IL1B-2060R | Recombinant Rhesus Macaque IL1B Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
IL1B-853HCL | Recombinant Human IL1B cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket