Recombinant Mouse C2 cell lysate

• Cat.No.: C2-001MCL
• Product Overview: Mouse C2 / Complement Component 2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.

Specification

• Source: Human cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: C2 complement component 2 (within H-2S) [ Mus musculus ]
• Official Symbol: C2
• Synonyms: C2; complement component 2 (within H-2S); complement C2; C3/C5 convertase; complement factor C2; MHC complement component C2; classical-complement pathway C3/C5 convertase
• Gene ID: 12263
• mRNA Refseq: NM_013484
• Protein Refseq: NP_038512
• Pathway: Activation of C3 and C5, organism-specific biosystem; Complement Activation, Classical Pathway, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Immune System, organism-specific biosystem
• Function: catalytic activity; hydrolase activity; peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity

Reviews

02/02/2023

The C2 protein comes highly recommended for its excellent performance in various research applications.

12/25/2020

It has demonstrated exceptional effectiveness in WB experiments, showcasing its reliability and precision in detecting and quantifying specific targets.

05/08/2019

Researchers can confidently rely on its capabilities to generate precise and significant results, enhancing the understanding of protein function and dynamics.

Q&As

Can C2 protein be a target for drug development in autoimmune disorders?

Yes, drugs targeting C2 or the complement system are being explored as potential treatments for autoimmune diseases to regulate the immune response.

Can C2 protein be used as a diagnostic marker for immune system disorders?

Yes, the absence or dysfunction of C2 protein can be indicative of immune system disorders, and its levels can be assessed for diagnostic purposes.

Can C2 protein be used in vaccine development?

Understanding C2's role in immune activation may contribute to vaccine design, potentially enhancing the effectiveness of vaccines by targeting the complement system.

How does C2 protein contribute to autoimmune diseases?

Abnormal activation of the complement system, including C2, is associated with some autoimmune diseases where the immune system mistakenly attacks the body's own cells.

Are there genetic factors influencing C2 protein levels and function?

Yes, genetic variations can affect C2 protein expression and function, leading to variations in immune response among individuals.