Recombinant Human Chromosome 12 Open Reading Frame 5, His-tagged

• Cat.No.: C12orf5-3371H
• Product Overview: Recombinant human TIGAR protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 32.6 kDa (294aa).

Specification

• Cat. No.: C12orf5-3371H
• Description: TIGAR, also known as TP53-induced glycolysis and apoptosis regulator, is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. These facts indicate that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses.
• Source: E.coli.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol.
• Purity: > 90% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MGSHMARFAL TVVRHGETRF NKEKIIQGQG VDEPLSETGF KQAAAAGIFL NNVKFTHAFS SDLMRTKQTM HGILERSKFC KDMTVKYDSR LRERKYGVVE GKALSELRAM AKAAREECPV FTPPGGETLD QVKMRGIDFF EFLCQLILKE ADQKEQFSQG SPSNCLETSL AEIFPLGKNH SSKVNSDSGI PGLAASVLVV SHGAYMRSLF DYFLTDLKCS LPATLSRSEL MSVTPNTGMS LFIINFEEGR EVKPTVQCIC MNLQDHLNGL TETR.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: C12orf5 chromosome 12 open reading frame 5 [ Homo sapiens ]
• Synonyms: chromosome 12 open reading frame 5; TIGAR; C12orf5; probable fructose-2,6-bisphosphatase TIGAR; transactivated by NS3TP2 protein; TP53-induced glycolysis and apoptosis regulator; EC 3.1.3.46
• Gene ID: 57103
• mRNA Refseq: NM_020375
• Protein Refseq: NP_065108
• MIM: 610775
• UniProt ID: Q9NQ88
• Chromosome Location: 12p13.3
• Function: fructose-2,6-bisphosphate 2-phosphatase activity; hydrolase activity