Recombinant Full Length Human ALG2 Protein, GST-tagged

• Cat.No.: ALG2-1432HF
• Product Overview: Human ALG2 full-length ORF ( NP_149078.1, 1 a.a. - 416 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 73.5 kDa
• Protein Length: 416 amino acids
• AA Sequence: MAEEQGRERD SVPKPSVLFL HPDLGVGGAE RLVLDAALAL QARGCSVKIW TAHYDPGHCF AESRELPVRC AGDWLPRGLG WGGRGAAVCA YVRMVFLALY VLFLADEEFD VVVCDQVSAC IPVFRLARRR KKILFYCHFP DLLLTKRDSF LKRLYRAPID WIEEYTTGMA DCILVNSQFT AAVFKETFKS LSHIDPDVLY PSLNVTSFDS VVPEKLDDLV PKGKKFLLLS INRYERKKNL TLALEALVQL RGRLTSQDWE RVHLIVAGGY DERVLENVEH YQELKKMVQQ SDLGQYVTFL RSFSDKQKIS LLHSCTCVLY TPSNEHFGIV PLEAMYMQCP VIAVNSGGPL ESIDHSVTGF LCEPDPVHFS EAIEKFIREP SLKATMGLAG RARVKEKFSP EAFTEQLYRY VTKLLV
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ALG2 asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: ALG2
• Synonyms: ALG2; asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S. cerevisiae); asparagine linked glycosylation 2 homolog (yeast, alpha 1,3 mannosyltransferase); alpha-1,3/1,6-mannosyltransferase ALG2; CDGIi; FLJ14511; hALPG2; NET38; homolog of yeast ALG2; alpha-1,3-mannosyltransferase ALG2; asparagine-linked glycosylation protein 2 homolog; GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase; GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase; GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase; asparagine-linked glycosylation 2 homolog (yeast, alpha-1,3-mannosyltransferase); asparagine-linked glycosylation 2 homolog (S. cerevisiae, alpha-1,3-mannosyltransferase)
• Gene ID: 85365
• mRNA Refseq: NM_033087
• Protein Refseq: NP_149078
• MIM: 607905
• UniProt ID: Q9H553

Reviews

07/20/2022

In immunological experiments, ALG2 was found to have good antigenicity and could induce target immune responses.

02/01/2021

The high solubility of ALG2 makes it easy to prepare the desired concentration.

09/03/2020

The high catalytic efficiency of ALG2 was found in enzymatic experiments.

11/23/2019

It was observed that ALG2 had better crystallinity, which was beneficial for crystallographic studies.

Q&As

Does ALG2 mutation have an adverse effect on embryonic development?

This mutations can have adverse effects on embryonic development and may therefore be associated with a number of fetal diseases.

What biological processes does ALG2 participate in?

ALG2 is involved in several biological processes including protein glycosylation, ER and Golgi body sugar metabolism.

Which cell signaling molecules are involved in ALG2?

ALG2 regulates the expression of glycosylase and the balance of glycosylation reaction by interacting with other glycosylated enzymes such as ALG3 and ALG1.

What are the effects of ALG2 inhibitors?

A number of inhibitors have been developed that target ALG2 and inhibit its glycosylation activity, thereby interfering with its function in cellular processes.

What are the action mechanisms of ALG2 ?

The catalyzes the sugar chain transfer reaction between UDP-glucose and substrate proteins, synthesizing sugar chains onto proteins.

Does ALG2 participate in mitochondrial glucose metabolism?

No studies have confirmed whether ALG2 is involved in mitochondrial glucose metabolism, and more studies are needed to prove it.

What non-cellular autophagy pathways are involved in ALG2?

Although there are currently no studies demonstrating the function of ALG2 in non-cellular autophagy pathways, there are indications that it may be involved in the TCA cycle and gluconeogenic pathways.

What are the molecular biological characteristics of ALG2?

ALG2 plays an important role in protein glycosylation pathway, participating in the synthesis and modification of sugar chains.

What effect does overexpression or knockout of ALG2 have on cells?

Overexpression or knockout of ALG2 can negatively affect cellular glycosylation and the stability of ER and Golgi bodies, but the specific effects need to be further explored.

What is the sugar chain selectivity of ALG2?

This protein selectively catalyzes the synthesis and modification of sugar chains and plays an important role in the binding selectivity of glycosylase family members to substrate proteins.

How is ALG2 distributed in mammalian cells?

ALG2 is mainly distributed in the endoplasmic reticulum and Golgi apparatus in mammalian cells.

In which cancers does ALG2 play a role?

At present, there is no direct evidence that ALG2 is related to the occurrence and development of cancer, but glycosylation plays an important role in metabolism and disease development, so it may be relevant to related diseases.

In what neurological diseases does ALG2 play a role?

There is currently no direct evidence that ALG2 is associated with neurological diseases, but glycosylation plays a key role in nervous system development and function, so there may be a correlation with neurological diseases.

What is the gene structure and regulation of ALG2?

This gene contains 10 exons, and its expression is influenced by multiple regulatory elements and signaling pathways.

Which place of ALG2 in cellular processes?

ALG2 is mainly distributed in the endoplasmic reticulum (ER) and the Golgi apparatus.

Which diseases do ALG2 mutations cause?

At present, there is no direct evidence that ALG2 mutations are directly related to some diseases, but glycosylation plays an important role in metabolism and disease development, so it may be related to related diseases.

What are the interacting proteins of ALG2?

The protein interacts with members of the glycosylase family such as ALG3, ALG1 and other related proteins to jointly regulate the glycosylation process of proteins.

In what metabolic diseases does ALG2 play a role?

There is no direct evidence that ALG2 is involved in the occurrence and development of metabolic diseases, but it may be associated with related metabolic diseases.