Recombinant Full Length Human C1QB Protein, C-Flag-tagged

• Cat.No.: C1QB-2136HFL
• Product Overview: Recombinant Full Length Human C1QB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the B-chain polypeptide of serum complement subcomponent C1q, which associates with C1r and C1s to yield the first component of the serum complement system. C1q is composed of 18 polypeptide chains which include 6 A-chains, 6 B-chains, and 6 C-chains. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. C1q deficiency is associated with lupus erythematosus and glomerulonephritis.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 26.5 kDa
• AA Sequence: MMMKIPWGSIPVLMLLLLLGLIDIS QAQLSCTGPPAIPGIPGIPGTPGPD GQPGTPGIKGEKGLPGLAGD HGEFGEKGDPGIPGNPGKVGPKGPM GPKGGPGAPGAPGPKGESGDYKATQ KIAFSATRTINVPLRRDQTI RFDHVITNMNNNYEPRSGKFTCKVP GLYYFTYHASSRGNLCVNLMRGRER AQKVVTFCDYAYNTFQVTTG GMVLKLEQGENVFLQATDKNSLLGM EGANSIFSGFLLFPDMEA TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus

Gene Information

• Gene Name: C1QB complement C1q B chain [ Homo sapiens (human) ]
• Official Symbol: C1QB
• Synonyms: complement component 1, q subcomponent, B chain; complement component 1, q subcomponent, beta polypeptide; complement component C1q, B chain; complement subcomponent C1q chain B; OTTHUMP00000002940
• Gene ID: 713
• mRNA Refseq: NM_000491.5
• Protein Refseq: NP_000482.3
• MIM: 120570
• UniProt ID: P02746

Reviews

02/13/2022

Product is worth the price.

09/09/2019

Outstanding performance in experiments.

04/03/2019

Excellent for biochemical assays.

Q&As

What role does C1QB play in opsonization?

C1QB, as part of C1q, plays a role in opsonization by marking pathogens and immune complexes for phagocytosis after binding and activating the complement system.

What is the primary function of the C1QB protein in the complement system?

C1QB is one of the chains (together with C1QA and C1QC) that forms the C1q molecule, which is the first component of the classical pathway of the complement system, playing a crucial role in innate immunity.

Are there any therapeutic implications related to targeting C1QB in diseases?

Given the role of C1QB in the complement system and its association with autoimmune diseases, targeting C1QB or its interactions might be a therapeutic strategy for conditions like SLE or other complement-mediated diseases.

What is the significance of C1QB in immune responses?

C1QB, as a part of the C1q molecule, plays a significant role in recognizing and binding to immune complexes, apoptotic cells, and certain pathogens, thereby activating the classical complement pathway and facilitating their clearance.

How does C1QB interact with other components of the complement system?

Once C1q (comprising C1QA, C1QB, and C1QC) binds to immune complexes, it triggers the binding and autoactivation of C1r and C1s serine proteases, forming the active C1 complex and initiating the classical complement cascade.

How does C1QB contribute to the formation of the C1 complex?

C1q is a hexamer formed by the association of six C1qA, six C1qB, and six C1qC chains. C1QB contributes to this hexameric structure, which is essential for the recognition of immune complexes.

Are there any known genetic mutations in the C1QB gene associated with diseases?

Mutations in the C1QB gene can lead to C1q deficiency, which is associated with an increased risk of systemic lupus erythematosus (SLE) and other autoimmune diseases.