Recombinant Full Length Human C3 Protein, C-Flag-tagged

• Cat.No.: C3-365HFL
• Product Overview: Recombinant Full Length Human C3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 187 kDa
• AA Sequence: MGPTSGPSLLLLLLTHLPLALGSPM YSIITPNILRLESEETMVLEAHDAQ GDVPVTVTVHDFPGKKLVLS SEKTVLTPATNHMGNVTFTIPANRE FKSEKGRNKFVTVQATFGTQVVEKV VLVSLQSGYLFIQTDKTIYT PGSTVLYRIFTVNHKLLPVGRTVMV NIENPEGIPVKQDSLSSQNQLGVLP LSWDIPELVNMGQWKIRAYY ENSPQQVFSTEFEVKEYVLPSFEVI VEPTEKFYYIYNEKGLEVTITARFL YGKKVEGTAFVIFGIQDGEQ RISLPESLKRIPIEDGSGEVVLSRK VLLDGVQNLRAEDLVGKSLYVSATV ILHSGSDMVQAERSGIPIVT SPYQIHFTKTPKYFKPGMPFDLMVF VTNPDGSPAYRVPVAVQGEDTVQSL TQGDGVAKLSINTHPSQKPL SITVRTKKQELSEAEQATRTMQALP YSTVGNSNNYLHLSVLRTELRPGET LNVNFLLRMDRAHEAKIRYY TYLIMNKGRLLKAGRQVREPGQDLV VLPLSITTDFIPSFRLVAYYTLIGA SGQREVVADSVWVDVKDSCV GSLVVKSGQSEDRQPVPGQQMTLKI EGDHGARVVLVAVDKGVFVLNKKNK LTQSKIWDVVEKADIGCTPG SGKDYAGVFSDAGLTFTSSSGQQTA QRAELQCPQPAARRRRSVQLTEKRM DKVGKYPKELRKCCEDGMRE NPMRFSCQRRTRFISLGEACKKVFL DCCNYITELRRQHARASHLGLARSN LDEDIIAEENIVSRSEFPES WLWNVEDLKEPPKNGISTKLMNIFL KDSITTWEILAVSMSDKKGICVADP FEVTVMQDFFIDLRLPYSVV RNEQVEIRAVLYNYRQNQELKVRVE LLHNPAFCSLATTKRRHQQTVTIPP KSSLSVPYVIVPLKTGLQEV EVKAAVYHHFISDGVRKSLKVVPEG IRMNKTVAVRTLDPERLGREGVQKE DIPPADLSDQVPDTESETRI LLQGTPVAQMTEDAVDAERLKHLIV TPSGCGEQNMIGMTPTVIAVHYLDE TEQWEKFGLEKRQGALELIK KGYTQQLAFRQPSSAFAAFVKRAPS TWLTAYVVKVFSLAVNLIAIDSQVL CGAVKWLILEKQKPDGVFQE DAPVIHQEMIGGLRNNNEKDMALTA FVLISLQEAKDICEEQVNSLPGSIT KAGDFLEANYMNLQRSYTVA IAGYALAQMGRLKGPLLNKFLTTAK DKNRWEDPGKQLYNVEATSYALLAL LQLKDFDFVPPVVRWLNEQR YYGGGYGSTQATFMVFQALAQYQKD APDHQELNLDVSLQLPSRSSKITHR IHWESASLLRSEETKENEGF TVTAEGKGQGTLSVVTMYHAKAKDQ LTCNKFDLKVTIKPAPETEKRPQDA KNTMILEICTRYRGDQDATM SILDISMMTGFAPDTDDLKQLANGV DRYISKYELDKAFSDRNTLIIYLDK VSHSEDDCLAFKVHQYFNVE LIQPGAVKVYAYYNLEESCTRFYHP EKEDGKLNKLCRDELCRCAEENCFI QKSDDKVTLEERLDKACEPG VDYVYKTRLVKVQLSNDFDEYIMAI EQTIKSGSDEVQVGQQRTFISPIKC REALKLEEKKHYLMWGLSSD
  FWGEKPNLSYIIGKDTWVEHWPEED ECQDEENQKQCQDLGAFTESMVVFG CPNTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus

Gene Information

• Gene Name: C3 complement C3 [ Homo sapiens (human) ]
• Official Symbol: C3
• Synonyms: ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
• Gene ID: 718
• mRNA Refseq: NM_000064.4
• Protein Refseq: NP_000055.2
• MIM: 120700
• UniProt ID: P01024