Recombinant Full Length Human CDK6 Protein, C-Flag-tagged

• Cat.No.: CDK6-790HFL
• Product Overview: Recombinant Full Length Human CDK6 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 36.8 kDa
• AA Sequence: MEKDGLCRADQQYECVAEIGEGAYG KVFKARDLKNGGRFVALKRVRVQTG EEGMPLSTIREVAVLRHLET FEHPNVVRLFDVCTVSRTDRETKLT LVFEHVDQDLTTYLDKVPEPGVPTE TIKDMMFQLLRGLDFLHSHR VVHRDLKPQNILVTSSGQIKLADFG LARIYSFQMALTSVVVTLWYRAPEV LLQSSYATPVDLWSVGCIFA EMFRRKPLFRGSSDVDQLGKILDVI GLPGEEDWPRDVALPRQAFHSKSAQ PIEKFVTDIDELGKDLLLKC
  LTFNPAKRISAYSALSHPYFQDLER CKENLDSHLPPSQNTSELNTATRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Small cell lung cancer

Gene Information

• Gene Name: CDK6 cyclin dependent kinase 6 [ Homo sapiens (human) ]
• Official Symbol: CDK6
• Synonyms: MCPH12; PLSTIRE
• Gene ID: 1021
• mRNA Refseq: NM_001259.8
• Protein Refseq: NP_001250.1
• MIM: 603368
• UniProt ID: Q00534

Reviews

07/06/2023

The results obtained after analysis by SDS-PAGE or Western blot were good.

03/14/2022

It has a wide range of applications and can be used in various biological experiments.

10/26/2020

The purity of CDK6 is good, and the experimental results obtained are very accurate.

Q&As

How is the expression level of CDK6 regulated?

The expression level of CDK6 is regulated by a variety of factors, including transcription factors, epigenetic modifications, and signal transduction pathways. These factors can affect the transcription and translation processes of the CDK6 gene, thereby regulating its expression level in cells.

What are the challenges in the development of CDK6 inhibitors?

There are several challenges in the development of CDK6 inhibitors, including issues such as inhibitor selectivity, drug resistance, and drug delivery. In addition, since CDK6 also has a certain function in normal cells, the side effects of inhibitors are also a factor to consider.

Does CDK6 have a role in stem cell biology?

It plays an important role in stem cell biology. It is involved in the process of self-renewal and differentiation of stem cells, as well as in determining the fate of stem cells. By regulating the activity of CDK6, it is possible to influence the proliferation and fate selection of stem cells.

What are the clinical applications of CDK6 inhibitors?

CDK6 inhibitors have been developed as potential anti-cancer drugs and have shown some efficacy in clinical trials. They block the progression of the cell cycle by inhibiting the activity of CDK6, thereby inhibiting the proliferation of cancer cells.

How is CDK6 activity regulated?

The activity of CDK6 is regulated at multiple levels, including binding to other cyclins and inhibitors, phosphorylation and dephosphorylation events, and transcriptional and post-translational modifications.

Does CDK6 function differently in different types of cancer?

The role of CDK6 in different types of cancer may vary. It may act as a promoter in some cancers and as a suppressant in others. This difference can be related to factors such as the type, stage, and genetic background of the cancer.