Recombinant Full Length Human RPA1 Protein, C-Flag-tagged

• Cat.No.: RPA1-685HFL
• Product Overview: Recombinant Full Length Human RPA1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 68 kDa
• AA Sequence: MVGQLSEGAIAAIMQKGDTNIKPIL QVINIRPITTGNSPPRYRLLMSDGL NTLSSFMLATQLNPLVEEEQ LSSNCVCQIHRFIVNTLKDGRRVVI LMELEVLKSAEAVGVKIGNPVPYNE GLGQPQVAPPAPAASPAASS RPQPQNGSSGMGSTVSKAYGASKTF GKAAGPSLSHTSGGTQSKVVPIASL TPYQSKWTICARVTNKSQIR TWSNSRGEGKLFSLELVDESGEIRA TAFNEQVDKFFPLIEVNKVYYFSKG TLKIANKQFTAVKNDYEMTF NNETSVMPCEDDHHLPTVQFDFTGI DDLENKSKDSLVDIIGICKSYEDAT KITVRSNNREVAKRNIYLMD TSGKVVTATLWGEDADKFDGSRQPV LAIKGARVSDFGGRSLSVLSSSTII ANPDIPEAYKLRGWFDAEGQ ALDGVSISDLKSGGVGGSNTNWKTL YEVKSENLGQGDKPDYFSSVATVVY LRKENCMYQACPTQDCNKKV IDQQNGLYRCEKCDTEFPNFKYRMI LSVNIADFQENQWVTCFQESAEAIL GQNAAYLGELKDKNEQAFEE
  VFQNANFRSFIFRVRVKVETYNDES RIKATVMDVKPVDYREYGRRLVMSI RRSALMTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Homologous recombination, Mismatch repair, Nucleotide excision repair

Gene Information

• Gene Name: RPA1 replication protein A1 [ Homo sapiens (human) ]
• Official Symbol: RPA1
• Synonyms: HSSB; RF-A; RP-A; REPA1; RPA70; MST075; PFBMFT6
• Gene ID: 6117
• mRNA Refseq: NM_002945.5
• Protein Refseq: NP_002936.1
• MIM: 179835
• UniProt ID: P27694

Reviews

04/20/2021

The background of RPA1 in Western blot was very clean and showed good antibody specificity.

02/09/2019

Beacuse reproducibility of RPA1 was very good and consistent results were obtained in each experiment, which gave me more confidence in the experimental results.

01/27/2019

RPA1 have a wide range of applications and can be used in various biological experiments.

Q&As

What is the importance of RPA1 protein in DNA recombination?

RPA1 protein is involved in important steps such as pairing, redox, and fusion in DNA recombination, and is necessary to effectively maintain genome integrity and stability.

What is the role of RPA1 protein in embryonic development?

RPA1 protein participates in DNA damage repair, growth and development in embryonic development, and has a role in maintaining the genome in embryos.

Can RPA1 protein be used to monitor or detect oxidative damage to DNA?

Since RPA1 protein plays an important role in DNA oxidative damage repair, it can be used as a marker of the degree of DNA oxidative damage to monitor and detect the risk of related diseases.

Is the RPA1 protein involved in DNA methylation modifications?

Although RPA1 protein is not a factor directly involved in DNA methylation modification, it may be involved in DNA methylation by participating in processes such as DNA damage repair, chromatin structure, and regulation of gene expression.

What role does RPA1 play in diabetes?

Studies have shown that RPA1 protein is involved in processes such as insulin secretion and regulation of β cell function in diabetes, and may become a new target for therapeutic strategies.

Is RPA1 protein involved in the immune system?

The RPA1 protein is involved in DNA damage repair and cell cycle in the immune system, and studies have found that it may be involved in processes such as immune response and pattern recognition.