Recombinant Full Length Mouse Gdp-Man:Man(3)Glcnac(2)-Pp-Dol Alpha-1,2-Mannosyltransferase(Alg11) Protein, His-Tagged

• Cat.No.: RFL32748MF
• Product Overview: Recombinant Full Length Mouse GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase(Alg11) Protein (Q3TZM9) (1-492aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Mus musculus (Mouse)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length (1-492)
• AA Sequence: MAADTGSWCVYAVLRFFYSLFFPGL MICGVLCVYLVIGLWVIRWHLQRKK KSVSTSKNGK EQTVVAFFHPYCNAGGGGERVLWCA LRALQKKYPEAVYVVYTGDINVSGQ QILDGAFRRF NIKLAHPVQFVFLRKRYLVEDSRYP HFTLLGQSLGSILLGWEALMQRVPD VYIDSMGYAF TLPLFKYVGGCRVGSYVHYPTISTD MLSVVKNQNPGFNNAAFISRNALLS KAKLIYYYLF AFVYGLVGSCSDIVMVNSSWTLNHI LSLWKVGHCTNIVYPPCDVQTFLDI PLHEKKVTPG HLLVSIGQFRPEKNHALQIKAFAKL LNEKAAELGHSLKLVLIGGCRNKDD EFRVNQLRSL SENLGVQENVEFKINISFDELKNYL SEATIGLHTMWNEHFGIGVVECMAA GTVILAHNSG GPKLDIVIPHEGQITGFLAESEEGY ADSMAHILSLSAEERLQIRKNARAS ISRFSDQEFE VAFLCSMEKLLT
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: Alg11
• Synonyms: Alg11; GDP-Man:Man(3GlcNAc(2-PP-Dol alpha-1,2-mannosyltransferase; Asparagine-linked glycosylation protein 11 homolog; Glycolipid 2-alpha-mannosyltransferase
• UniProt ID: Q3TZM9

Gene Information

• Gene Name: Alg11
• Synonyms: Alg11; GDP-Man:Man(3GlcNAc(2-PP-Dol alpha-1,2-mannosyltransferase; Asparagine-linked glycosylation protein 11 homolog; Glycolipid 2-alpha-mannosyltransferase
• UniProt ID: Q3TZM9

Reviews

10/06/2022

Widely available and easy to prepare.

07/21/2022

Easy to store, more stable.

04/30/2021

Compared with other methods, qPCR detection is better.

05/26/2019

Easy to purify in production, high purity.

Q&As

Is there a correlation between ALG11 defects and neurological diseases?

Some ALG11 mutations may lead to central nervous system diseases, such as cerebral cortex atrophy, etc. However, a large number of studies are needed to support the adequacy and validity of the above relationship.

How is the effect of ALG11 defect on the cellular life activity process produced?

The effects of ALG11 defects on cell life activities may include metabolic abnormalities, protein synthesis and secretion abnormalities, etc. The mechanism of these effects needs to be further explained.

What is the relationship between ALG11 gene family structure and evolution?

ALG11 is part of the GMD family and is found in winged animals and other eukaryotes. More studies are needed to confirm the family structure and evolutionary relationship.

How to evaluate the clinical manifestations of ALG11-related synthase deficiency syndrome?

The clinical manifestations of patients with ALG11 deficiency syndrome are complicated, and it is necessary to synthesize various evidences, such as clinical symptoms, magnetic resonance, nuclear magnetic resonance, X-ray and other comprehensive evaluation, to determine the specific disease type.

How to screen ALG11 gene function?

The functional screening of ALG11 gene needs to be based on the special characteristics of its genome sequence and encoded protein, combined with the existing research results, and adopt appropriate screening technology to determine.

How specific is the glycosylase matrix of ALG11 gene?

The relationship between ALG11 gene and specific matrix substances in the glycosylation process is not completely clear, and further research is needed.

How to evaluate the effect of abnormal function of ALG11 gene on the development and reproduction of organisms?

At present, the effects of ALG11 gene defects on the development and reproduction of organisms still need to be explored.

What is the effect of ALG11 mutation on protein glycosylation?

At present, the mechanism of the effect of ALG11 gene mutation on protein glycosylation needs to be studied at the cellular level to elucidate a comprehensive influence model.

How does ALG11 mediate cell membrane regeneration?

ALG11 may be involved in cell membrane regeneration through the modification of membrane glycoproteins, glycolipids, peptides and other molecules.

Are there known quantitative ALG11 gene polymorphisms associated with other genetic or environmental factors?

There is no evidence that ALG11 polymorphisms are associated with other genetic or environmental factors. But research is continuing.

What is the genomic structure and regulatory mechanism of ALG11?

The ALG11 gene is composed of multiple exons that were included in previous genomic sequencing studies. The regulation mechanism still needs to be studied further.

Are ALG11 gene mutations associated with specific diseases?

ALG11 gene mutations may be associated with heart disease and other syndromes associated with protein glycosylation. But more research is needed to confirm the evidence.

What is the influence mechanism of ALG11 defect on lipid metabolism?

ALG11 defects may lead to lipid metabolism abnormalities by modifying protein modifications related to lipid metabolism. However, further studies are needed to determine the mechanism.

How to evaluate ALG11 expression and glycosylation to analyze the therapeutic mechanism of Cadoria?

At present, there are not enough research results on how ALG11 affects the therapeutic mechanism of Cardoria, and more data are needed to support it in the future.

What are the unique characteristics of ALG11 expression patterns and post-transcriptional modifications?

The post-transcriptional modification and expression patterns of ALG11 gene require more biochemical studies to further explain.

In which cell types or tissues is ALG11 gene highly expressed?

The expression of ALG11 gene in different tissues and cell types may vary, please refer to relevant literature.

Does the influence mechanism of ALG11 defect on the regulation level of gene expression?

The mechanism of ALG11 defect and its effect on glycosylation exist a series of complex regulatory mechanisms, which need to be further studied at the molecular and cellular levels.

What are the structural and functional characteristics of ALG11 encoded protein?

The ALG11 encoding protein is involved in the process of protein glycosylation, and the details such as what mechanism achieves this process are still in the field of unstudied.

How to prevent regional mutation and sequence variation of ALG11 gene?

To prevent the regional mutation or sequence variation of ALG11 gene, it is necessary to strengthen the collection and preservation of relevant biological samples, as well as perfect data management and maintenance systems.

How does ALG11 affect the occurrence and progression of lung lesions?

More research is needed on the association between the ALG11 gene and lung disease, which has only been a concern in recent years.