Recombinant Full Length Mouse Lysosomal Acid Phosphatase(Acp2) Protein, His-Tagged

• Cat.No.: RFL-2142MF
• Product Overview: Recombinant Full Length Mouse Lysosomal acid phosphatase(Acp2) Protein (P24638) (31-423aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Mus musculus (Mouse)
• Tag: His
• Form: Lyophilized powder
• Protein length: Full Length (31-423)
• AA Sequence: RSLRFVTLLYRHGDRSPVKTYPKDP YQEEKWPQGFGQLTKEGMLQHWELG QALRQRYHGFLNTSYHRQEVYVRST DFDRTLMSAEANLAGLFPPNEVQHF NPNISWQPIPVHTVPITEDRLLKFP LGPCPRYEQLQNETRQTPEYQNRSI QNAQFLNMVANETGLTNVTLETIWN VYDTLFCEQTHGLLLPPWASPQTVQ RLSQLKDFSFLFLFGIHEQVQKARL QGGVLLAQILKNLTLMATTSQFPKL LVYSAHDTTLVALQMALNVYNGKQA PYASCHIFELYQEDNGNFSVEMYFR NDSKKAPWPLILPGCPHRCPLQDFL RLTEPVIPKDWQKECQLANDTADTE VIVALAVCGSILFLLIVLLLTILFR MQAQPPGYHHVADREDHA
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: SDS-PAGE
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: Acp2
• Synonyms: Acp2; Lysosomal acid phosphatase; LAP
• UniProt ID: P24638

Gene Information

• Gene Name: Acp2
• Synonyms: Acp2; Lysosomal acid phosphatase; LAP
• UniProt ID: P24638

Reviews

05/06/2021

Highly recommended!

10/02/2019

Great performance in ELISA.

05/02/2018

Excellent, we will definitely go back to buy this again!

04/23/2018

Fast delivery !

04/22/2018

Satisfied with five starts!

Q&As

What is the buffer solution of the ACP2?

100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

One study found the downregulation of ACP2 expression could significantly induce the HCT-116 and DLD1 cells to survive 5-FU treatment compared with control. What does this conlcusion imply?

The high expression of ACP2 in CRC patients was associated with increased sensitivity to 5-FU chemotherapy.

Could please simply descibe the post-translational modifications for ACP2 gene?

The membrane-bound form is converted to the soluble form by sequential proteolytic processing; First, the C-terminal cytoplasmic tail is removed; Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen.

What will happen if the enzyme inactive occur in this gene?

An enzymatically-inactive allele of this gene in mice showed severe growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype.

What is the preformance in mice if ACP2 was deleted?

Mice lacking this gene showed multiple defects, including bone structure alterations, lysosomal storage defects, and an increased tendency towards seizures.

What you can discover if protein expression of lysosomal acid phosphatase 2 (ACP2) was increased in colorectal cancer among patients received chemotherapy?

Among patients who received chemotherapy, those with an high expression of ACP2 showed better survival in both stage II and III colorectal cancer than those with low ACP2 expression.

List 2 inferred drugs for ACP2 gene, and their status?

D-Phenylalanine - Approved, Experimental, Investigational. D-Tyrosine - Approved, Experimental, Investigational.

What is the formulation of the ACP2 protein solution?

ACP2 protein solution (1mg/ml) contains 20mM Tris-HCl (pH8.0) and 10% glycerol.

What is the stability of the ACP2?

Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

What are the top5 interacting proteins with ACP2 gene?

They are TRHR, GMDS, SGCA, FPGS, and BAZ1B