Recombinant Human ACADM Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ACADM-1433H
• Product Overview: ACADM MS Standard C13 and N15-labeled recombinant protein (NP_000007) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 46.6 kDa
• AA Sequence: MAAGFGRCCRVLRSISRFHWRSQHT KANRQREPGLGFSFEFTEQQKEFQA TARKFAREEIIPVAAEYDKTGEYPV PLIRRAWELGLMNTHIPENCGGLGL GTFDACLISEELAYGCTGVQTAIEG NSLGQMPIIIAGNDQQKKKYLGRMT EEPLMCAYCVTEPGAGSDVAGIKTK AEKKGDEYIINGQKMWITNGGKANW YFLLARSDPDPKAPANKAFTGFIVE ADTPGIQIGRKELNMGQRCSDTRGI VFEDVKVPKENVLIGDGAGFKVAMG AFDKTRPVVAAGAVGLAQRALDEAT KYALERKTFGKLLVEHQAISFMLAE MAMKVELARMSYQRAAWEVDSGRRN TYYASIAKAFAGDIANQLATDAVQI LGGNGFNTEYPVEKLMRDAKIYQIY EGTSQIQRLIVAREHIDKYKNTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ACADM acyl-CoA dehydrogenase medium chain [ Homo sapiens (human) ]
• Official Symbol: ACADM
• Synonyms: ACADM; acyl-CoA dehydrogenase, C-4 to C-12 straight chain; acyl Coenzyme A dehydrogenase, C 4 to C 12 straight chain; medium-chain specific acyl-CoA dehydrogenase, mitochondrial; ACAD1; MCAD; MCADH; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• Gene ID: 34
• mRNA Refseq: NM_000016
• Protein Refseq: NP_000007
• MIM: 607008
• UniProt ID: P11310

Reviews

04/29/2022

Demonstrates excellent stability and consistency in long-term storage.

05/25/2018

Highly effective in detecting post-translational modifications of the target protein.

Q&As

How does the activity of this protein contribute to cellular metabolism?

The activity of acyl-CoA dehydrogenase medium chain contributes to the breakdown of medium-chain fatty acids to produce energy in cells.

Are there any known inhibitors or activators of this protein?

There are currently no known inhibitors or activators of acyl-CoA dehydrogenase medium chain protein.

What research is currently being conducted to further understand the function of acyl-CoA dehydrogenase medium chain protein?

Research is ongoing to further understand the function and regulation of acyl-CoA dehydrogenase medium chain protein, as well as its role in metabolic disorders.

What diseases or disorders are associated with mutations in this protein?

Mutations in this protein are associated with a variety of metabolic disorders, including medium-chain acyl-CoA dehydrogenase deficiency.

How is the expression of acyl-CoA dehydrogenase medium chain regulated?

The expression of acyl-CoA dehydrogenase medium chain is regulated by a variety of factors, including gene expression and protein degradation pathways.

Where is acyl-CoA dehydrogenase medium chain protein located within the cell?

Acyl-CoA dehydrogenase medium chain protein is located in the mitochondrial matrix.

What is the substrate specificity of this protein?

Acyl-CoA dehydrogenase medium chain protein has specificity for medium-chain fatty acyl-CoA substrates.

What is the structure of this protein and how does it relate to its function?

Acyl-CoA dehydrogenase medium chain protein has a flavin adenine dinucleotide (FAD)-binding domain that is essential for its function.

What is the function of acyl-CoA dehydrogenase medium chain protein?

Acyl-CoA dehydrogenase medium chain protein catalyzes the first step in the 尾-oxidation of medium-chain fatty acids.

What are the physiological roles of acyl-CoA dehydrogenase medium chain?

Acyl-CoA dehydrogenase medium chain plays a key role in energy metabolism and fatty acid oxidation in cells.