Recombinant Human ADCK4 293 Cell Lysate

• Cat.No.: ADCK4-9022HCL

Specification

• Description: Antigen standard for aarF domain containing kinase 4 (ADCK4), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADCK4 aarF domain containing kinase 4 [ Homo sapiens ]
• Official Symbol: ADCK4
• Synonyms: ADCK4; aarF domain containing kinase 4; uncharacterized aarF domain-containing protein kinase 4; COQ8; FLJ12229
• Gene ID: 79934
• mRNA Refseq: NM_001142555
• Protein Refseq: NP_001136027
• UniProt ID: Q96D53
• Chromosome Location: 19q13.2
• Function: kinase activity; protein serine/threonine kinase activity

Q&As

Can ADCK4 protein be used to develop new drugs?

While there are currently no specific drugs targeting ADCK4 protein, the regulation of the CoQ10 biosynthesis pathway and its involvement in various diseases suggest that ADCK4 protein may be a useful target for drug development. Compounds that can increase CoQ10 biosynthesis or protect against oxidative stress may be potential therapeutic agents for diseases associated with CoQ10 deficiency.

Are there any clinical trials for ADCK4 protein therapy?

Currently, there are no clinical trials for ADCK4 protein therapy, but research is ongoing to explore the potential therapeutic applications of ADCK4 protein or the CoQ10 biosynthesis pathway. It is possible that clinical trials may be developed in the future, particularly for diseases that involve mitochondrial dysfunction.

Are there any clinical trials using ADCK4 protein or CoQ10 as a treatment?

There are currently ongoing clinical trials investigating the use of CoQ10 in the treatment of different diseases, including primary and secondary CoQ10 deficiencies, Parkinson's disease, and heart failure. However, there are no clinical trials specifically using ADCK4 protein as a treatment.

Are there any dietary interventions that can affect ADCK4 protein levels?

While there is currently little research on the effects of dietary interventions on ADCK4 protein levels specifically, it is known that a diet rich in fruits and vegetables, which are high in antioxidants, can help reduce oxidative stress and protect against CoQ10 deficiency. Therefore, a healthy diet may help support the function of the CoQ10 biosynthesis pathway.

Can ADCK4 protein be used as a biomarker for mitochondrial dysfunction?

While ADCK4 protein is involved in CoQ10 biosynthesis and mitochondrial function, it is not currently used as a biomarker for mitochondrial dysfunction. Other markers, such as ATP levels, mitochondrial DNA mutations, and reactive oxygen species, are more commonly used to diagnose mitochondrial dysfunction. However, research is ongoing to explore the potential use of ADCK4 protein as a biomarker.

What are the potential applications of ADCK4 protein?

ADCK4 protein is involved in the biosynthesis of coenzyme Q10 (CoQ10), which plays a critical role in cellular energy production and the regulation of oxidative stress. Therefore, ADCK4 protein may have potential applications in the development of new therapies for diseases associated with CoQ10 deficiency, such as primary and secondary CoQ10 deficiencies, nephrotic syndrome, and other neurological disorders.

Is ADCK4 protein being developed as a potential therapeutic target for any diseases?

Currently, there are no specific drugs or therapies that target ADCK4 protein, but research is ongoing to explore its potential therapeutic applications. Diseases that involve mitochondrial dysfunction, such as Parkinson's disease, may benefit from therapies that target ADCK4 protein or the CoQ10 biosynthesis pathway. However, more research is needed to understand the role of ADCK4 in these diseases.

Can ADCK4 protein be used to improve cognitive function?

While ADCK4 protein plays a role in cellular energy production and mitochondrial function, there is no evidence to suggest that ADCK4 protein supplementation can improve cognitive function. However, CoQ10 supplements have been studied for their potential benefits for cognitive function and neurological disorders, and further research is needed to understand the potential role of ADCK4 protein in these conditions.

Can ADCK4 protein supplementation be used to improve athletic performance?

There is no evidence to suggest that ADCK4 protein supplementation can improve athletic performance. While CoQ10 supplements have been studied for their potential benefits for athletes, the effects of ADCK4 protein supplementation are not yet understood.

Can exercise affect ADCK4 protein levels?

There is currently limited research on the effects of exercise on ADCK4 protein levels specifically. However, exercise has been shown to improve mitochondrial function and reduce oxidative stress, which are important factors in the regulation of CoQ10 biosynthesis. Therefore, exercise may indirectly affect ADCK4 protein levels by improving overall mitochondrial function and reducing oxidative stress.

Are there any side effects associated with ADCK4 protein or CoQ10 supplementation?

While CoQ10 supplementation is generally considered safe and well-tolerated, some people may experience mild side effects such as nausea, vomiting, and gastrointestinal upset. CoQ10 may also interact with certain medications, such as blood thinners and chemotherapy drugs. However, there is currently no evidence to suggest that ADCK4 protein supplementation would have any adverse side effects.

Is there a diagnostic test for ADCK4 mutations?

Yes, genetic testing can be used to identify mutations in the ADCK4 gene that are associated with hereditary glomerulopathies and other diseases. Genetic testing can be used to diagnose and monitor these conditions, as well as to identify carriers of ADCK4 mutations who may be at risk for passing on the mutation to their children.

Can ADCK4 protein be used in cosmetic products?

There is currently no evidence to suggest that ADCK4 protein would have any cosmetic benefits. While CoQ10 is sometimes used in cosmetic products due to its antioxidant properties, it is unclear whether the addition of ADCK4 protein would have any added benefits.