Recombinant Human ALG1 293 Cell Lysate

• Cat.No.: ALG1-8909HCL

Specification

• Description: Antigen standard for asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae) (ALG1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ALG1 asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: ALG1
• Synonyms: ALG1; asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae); asparagine linked glycosylation 1 homolog (yeast, beta 1,4 mannosyltransferase); chitobiosyldiphosphodolichol beta-mannosyltransferase; HMAT1; HMT 1; mannosyltransferase-1; beta-1,4 mannosyltransferase; beta-1,4-mannosyltransferase; GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase; asparagine-linked glycosylation protein 1 homolog; GDP-mannose-dolichol diphosphochitobiose mannosyltransferase; asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase); HMT1; MT-1; CDG1K; HMT-1; Mat-1; hMat-1
• Gene ID: 56052
• mRNA Refseq: NM_019109
• Protein Refseq: NP_061982
• MIM: 605907
• UniProt ID: Q9BT22
• Chromosome Location: 16p13.3
• Pathway: Asparagine N-linked glycosylation, organism-specific biosystem; Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; N-Glycan biosynthesis, organism-specific biosystem; N-Glycan biosynthesis, conserved biosystem; N-glycan precursor biosynthesis, organism-specific biosystem
• Function: chitobiosyldiphosphodolichol beta-mannosyltransferase activity; mannosyltransferase activity; transferase activity, transferring glycosyl groups

Reviews

02/24/2022

The protein specificity is high and the misdiagnosis rate is low.

12/22/2020

The most suitable detection method is ELISA.

12/15/2020

High sensitivity in diagnosis.

04/13/2020

Detection limit is low, low content can also be successfully detected。

Q&As

What is the ALG1 test fee?

ALG1 testing costs vary by hospital and region and may fluctuate within a range.

Does ALG1 detection require special preparation and precautions?

ALG1 testing does not require special preparation before it is performed, but if a blood sample needs to be collected, the blood collection process needs to be completed under the guidance of a doctor.

What groups of people are more susceptible to ALG1 defects?

ALG1 deficiency is a genetic disorder, so it may affect members of its family.

How is ALG1 defect caused?

ALG1 defects are usually caused by mutations, loss or deletion of genes, which in turn affect the metabolism of protein glycosylation.

Does ALG1 defect affect fertility?

ALG1 defects may affect fertility and can cause problems such as genetic disorders.

What is the role of ALG1 in the human body?

ALG1 is an important gene involved in protein glycosylation in human body, and plays an important role in maintaining the normal function of human body.

Is ALG1 testing routine?

Testing is not a routine procedure and is usually done on the advice of a doctor.

What factors interfere with ALG1 detection?

Detection can be interfered with by a number of factors, such as clinical symptoms, age, gender, diet, and so on.

Is there a familial risk of ALG1 deficiency?

Deficiency has a familial genetic risk, and the risk of developing the disease depends on the family genetic history.

How to interpret the test results of ALG1?

ALG1 test results need to be interpreted and analyzed by a professional.

Does the ALG1 test require a professional?

ALG1 testing is performed by a professional clinical geneticist and laboratory technician.

What diseases is the ALG1 test mainly used to detect?

ALG1 detection is mainly used to detect CDG1K and other related diseases.

Can ALG1 defects be inherited?

ALG1 deficiency is a genetic disorder that can be passed on to future generations through genes.

What Clinical application ALG1 tested for?

This protein can be tested in the form of blood or oral swabs.

Does ALG1 test require fasting?

Testing does not require fasting.

What diseases can ALG1 defect cause?

Deficiency of ALG1 may cause a rare glycoprotein Disorder called Glycosylation 1k syndrome (CDG1K), which includes mental retardation, motor degeneration, and muscle relaxation.

What are the symptoms of ALG1 defect?

Deficiency may cause mental retardation, motor function degradation, fluid on the head, eye problems and other symptoms.

How is ALG1 defect treated?

Deficiency can be treated with targeted treatment for its clinical manifestations, such as symptom relief, rehabilitation, etc.

Can ALG1 test protect against disease?

Testing may help in the early detection of related diseases, so that targeted prevention and treatment measures can be taken, but often cannot prevent all related diseases.