Recombinant Human ALG12, GST-tagged

• Cat.No.: ALG12-9572H
• Product Overview: Recombinant Human ALG12 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: This gene encodes a member of the glycosyltransferase 22 family. The encoded protein catalyzes the addition of the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation. Mutations in this gene have been associated with congenital disorder of glycosylation type Ig (CDG-Ig)characterized by abnormal N-glycosylation.
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: 375-488a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: ALG12 asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: ALG12
• Synonyms: ALG12; asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae); asparagine linked glycosylation 12 homolog (yeast, alpha 1,6 mannosyltransferase); dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase; ECM39; membrane protein SB87; mannosyltransferase ALG12 homolog; asparagine-linked glycosylation protein 12 homolog; dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichyl-alpha-1,6-mannosyltransferase; dolichyl-P-mannose:Man-7-GlcNAc-2-PP-dolichyl-alpha-6-mannosyltransferase; asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase); asparagine-linked glycosylation 12 homolog (S. cerevisiae, alpha-1,6-mannosyltransferase); CDG1G; hALG12; PP14673; MGC3136; MGC111358
• Gene ID: 79087
• mRNA Refseq: NM_024105
• Protein Refseq: NP_077010
• MIM: 607144
• UniProt ID: Q9BV10
• Chromosome Location: 22q13.33
• Pathway: Asparagine N-linked glycosylation, organism-specific biosystem; Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; N-Glycan biosynthesis, organism-specific biosystem; N-Glycan biosynthesis, conserved biosystem; N-glycan precursor biosynthesis, organism-specific biosystem
• Function: alpha-1,6-mannosyltransferase activity; transferase activity, transferring glycosyl groups

Reviews

10/26/2020

An important metabolic enzyme in the pathway of polysaccharide metabolism and pentose metabolism.

05/31/2020

It can be used as a biomarker to prevent disease.

01/19/2020

Strong operability in the experiment.

02/14/2019

Activity is specific.

Q&As

How do ALG12 defects affect cell signaling mechanisms?

ALG12 defects have not yet been fully explained by research on how they affect cell signaling mechanisms.

Are there known quantitative ALG12 gene polymorphisms associated with other genetic or environmental factors?

The relationship between ALG12 gene polymorphism and other genetic or environmental factors is still insufficient, and the existing results are not enough to make a full proof.

What are the clinical features and prognostic markers of ALG12 mutation?

This protein mutations can lead to the failure of the synthesis of polysaccharides and glycoproteins, leading to diseases such as glycogen disease. Its clinical characteristics need to be evaluated through clinical studies.

How to specify and function the code reading frame of ALG12?

ALG12's reading frame specifies an annotated sequence of organic molecules, branching point reactions in the process of breaking down sugars and lipids.

What are the structural and functional characteristics of ALG12's coding protein?

The protein encoded by ALG12 is involved in the reactants of the transfer chemical reaction, enabling the successful formation of polysaccharides and glycoproteins. Its functional characteristics are related to glycosyl synthesis in other glycogen organisms.

In which tissues does ALG12 exist?

ALG12 is expressed in different tissues in different amounts, such as in fat, liver, gastrointestinal tract and muscle.

How does ALG12 affect the interaction between fatty acid metabolism and glucose metabolism?

ALG12 is involved in the catalysis of glycosylation process, which may affect the interaction of fatty acid and glucose metabolism, but more studies are needed to clarify.

What are the regulatory mechanisms of ALG12 expression and related signaling pathways?

The mechanism and signaling pathway of ALG12 gene regulation are similar to other glycosylation genes, but the specific situation remains to be studied.

How to study the effect of ALG12 mutation on the nervous system?

The mutations may cause a variety of neurological diseases and require further research using relevant animal models and nervous system cell lines.

What are the unique characteristics of ALG12 expression patterns and post-transcriptional modifications?

The post-transcriptional modification and expression pattern of ALG12 gene have not been studied deeply enough, and further research is needed.

What is the special regulatory mechanism of ALG12 as a branching enzyme?

This can be used as a branch enzyme of NSDHL, if it produces an anthraquinone derivative with progesterone, and increases the amount of activated enzyme by reversing the reaction; It can also be treated with hyperliquefied P-body enzymes.

How is the association of ALG12 gene mutation with disease assessed?

At present, a large number of studies have been carried out on the correlation between gene mutation and ALG12 gene and diseases, and many diseases have been confirmed to be associated with ALG12 gene mutation, but these scientific research results need more verification and analysis.

What is the mechanism of the effect of ALG12 mutation on lipid metabolism and enzyme activity?

ALG12 mutations may affect lipid metabolism and enzyme activity, and there are some studies and confirmations, and further studies are needed to determine the specific mechanisms.

How does ALG12 regulate energy metabolism through glucose and fat synthesis?

Although ALG12 has a regulatory role in lipid and glycogen processing, its detailed mechanism of action needs to be further investigated in cell and animal models.

What is the genomic structure and regulatory mechanism of ALG12?

The ALG12 genome structure is composed of multiple exons, which need to be processed into unique mRNA after transcription, and then function through regulation by various regulatory mechanisms.

Does ALG12 mutation have an effect on tolerance to hypoxia?

Until now, there have been few studies on the effect of ALG12 mutation on hypoxia tolerance.

Does the relationship between the enzyme encoded by ALG12 and protein glycosylation?

ALG12 encodes proteins that extend chains by transferring acid groups through molecules in glycosylation catalyzed by proteins such as lactase and glycoprotein processing enzymes.

Are mutations in ALG12 associated with specific diseases?

ALG12 mutations may be associated with disorders of lipid metabolism and neurological disorders.