Recombinant Human ALG13, His-tagged

• Cat.No.: ALG13-9573H
• Product Overview: Recombinant Human ALG13 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Description: The protein encoded by this gene is a subunit of a bipartite UDP-N-acetylglucosamine transferase. It heterodimerizes with asparagine-linked glycosylation 14 homolog to form a functional UDP-GlcNAc glycosyltransferase that catalyzes the second sugar addition of the highly conserved oligosaccharide precursor in endoplasmic reticulum N-linked glycosylation. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: E.coli
• Species: Human
• Tag: His
• Protein length: 1-165a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: ALG13 asparagine-linked glycosylation 13 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: ALG13
• Synonyms: ALG13; asparagine-linked glycosylation 13 homolog (S. cerevisiae); chromosome X open reading frame 45 , CXorf45, GLT28D1, glycosyltransferase 28 domain containing 1; UDP-N-acetylglucosamine transferase subunit ALG13 homolog; FLJ23018; MDS031; YGL047W; glycosyltransferase 28 domain-containing protein 1; hematopoietic stem/progenitor cells protein MDS031; CXorf45; GLT28D1; FLJ31785; MGC12423
• Gene ID: 79868
• mRNA Refseq: NM_001039210
• Protein Refseq: NP_001034299
• MIM: 300776
• UniProt ID: Q9NP73
• Chromosome Location: Xq23
• Pathway: Asparagine N-linked glycosylation, organism-specific biosystem; Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; N-Glycan biosynthesis, organism-specific biosystem; N-Glycan biosynthesis, conserved biosystem; N-glycan precursor biosynthesis, organism-specific biosystem
• Function: N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity; carbohydrate binding; transferase activity, transferring hexosyl groups

Reviews

01/13/2023

Strong antioxidant effect, can inhibit the formation of free radicals, reduce oxidation reaction.

06/18/2021

The clinical detection method is mature.

01/08/2019

Great performance in ELISA.

Q&As

How does the protein encoded by ALG13 gene transmit catalytic activity?

The protein encoded by the ALG13 gene catalyzes its receptor in an acylated manner, making it a substrate for glycosyltransferase, thereby transmitting its catalytic activity.

What neurological diseases are ALG13 mutations associated with?

The mutations have been linked to neurological disorders such as idiopathic epilepsy and intellectual disability.

How can ALG13 gene variants be assessed for genetic risk of neurological diseases?

Appropriate genetic analysis methods, including single genotypic/association analysis and genome-linked DNA polymorphism analysis, can be used to assess the genetic risk of ALG13 gene variation and neurological diseases.

What is the current status of research on the correlation between ALG13 mutation and cardiovascular disease and metabolic syndrome?

At present, there are not enough studies to prove that ALG13 mutations are directly related to diseases such as cardiovascular disease and metabolic syndrome.

Which gene family does ALG13 belong to?

ALG13 is a member of the UDP-glucose-glycosyltransferase family, which is mainly involved in protein glycosylation.

Is the ALG13 gene related to human evolutionary history?

The ALG13 gene has not been linked to the evolutionary history of humans.

Does ALG13 mutation affect neuronal development and nerve conduction?

This mutations may affect neuronal development and nerve conduction processes, leading to a range of neurological diseases.

How to explore the function of ALG13 gene?

CRISPR/Cas9 gene editing technology can be used to construct ALG13 gene knockout or mutant cell lines, and its effects on cell growth and glycosylation process can be analyzed through comparative experiments.

Is the regulatory mechanism of ALG13 related to AMPK, mTOR and other pathways?

No studies have directly linked ALG13 with AMPK and mTOR, and further research is needed.

What is the potential role of ALG13 in the early prediction and treatment of diabetes?

The potential role of ALG13 in the early prediction and treatment of diabetes has not been studied, which requires further research.

How to study the relationship between ALG13 and diseases related to glucose metabolism such as diabetes?

In this study, we can use gene editing technology to construct ALG13 gene knockout or mutant cell lines, and then compare the differences in glucose metabolism and energy metabolism between them and wild-type cells.

Is there a correlation between ALG13 metabolism and obesity?

There is currently no direct evidence of a correlation between ALG13 and metabolism and obesity.

What is the regulatory mechanism of ALG13 on glucose level and energy metabolism?

At present, there is no conclusive evidence that ALG13 has a direct regulatory effect on glucose levels and energy metabolism, but genomic studies suggest that it may be involved in the mechanisms of glucose metabolism pathways.

How does ALG13 mutation affect protein glycosylation?

ALG13 encodes an important glycosyltransferase, and its mutation can affect the glycosylation process of proteins, and thus affect the efficiency and quality of glycosylation.

How does glycosylation of ALG13 affect protein function?

Glycosylation is one of the important ways of protein modification, and the glycosylation of ALG13 can affect the folding, activity and stability of the protein, so as to affect the function of the protein.

How is the enzyme activity of ALG13 regulated?

The enzyme activity of ALG13 is controlled by various regulatory mechanisms. One is to interact with the sugar chain component glycosyl budding to regulate its activity and stability; The other is its own acylation modification, which affects the activity.

What is the unique mechanism of ALG13 in terms of neuronal papillary process message transmission?

ALG13 is involved in metastasis as glycosylation and affects neuronal papillary process signaling, etc. Further studies are needed to understand its unique mechanism.

Are there known ALG13 gene polymorphisms associated with other genetic or environmental factors?

The relationship between ALG13 gene polymorphism and other genetic or environmental factors is limited and further research is needed.

What are the different expression patterns of ALG13 in different tissues?

The amount and pattern of ALG13 expression in different tissues are different, such as high expression in brain, lung and kidney.