Recombinant Human ANO1 Protein, His-tagged
Cat.No. : | ANO1-156H |
Product Overview : | Recombinant Human ANO1 Protein (Met1-Ala333) with N-His tag was expressed in E. coli. |
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Description : | Enables calcium activated cation channel activity; intracellular calcium activated chloride channel activity; and iodide transmembrane transporter activity. Involved in cation transport; inorganic anion transport; and positive regulation of insulin secretion involved in cellular response to glucose stimulus. Located in apical plasma membrane and nucleoplasm. |
Source : | E. coli |
Species : | Human |
Tag : | N-His |
Form : | Freeze-dried powder |
Molecular Mass : | Predicted Molecular Mass: 42.2 kDa Accurate Molecular Mass: 44 kDa |
Protein length : | Met1-Ala333 |
Purity : | > 80% |
Applications : | Positive Control; Immunogen; SDS-PAGE; WB. |
Stability : | The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months. |
Storage Buffer : | 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
Reconstitution : | Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name : | ANO1 anoctamin 1, calcium activated chloride channel [ Homo sapiens (human) ] |
Official Symbol : | ANO1 |
Synonyms : | ANO1; anoctamin 1, calcium activated chloride channel; oral cancer overexpressed 2, ORAOV2, TMEM16A, transmembrane protein 16A; anoctamin-1; DOG1; FLJ10261; TAOS2; oral cancer overexpressed 2; tumor-amplified and overexpressed sequence 2; discovered on gastrointestinal stromal tumors protein 1; transmembrane protein 16A (eight membrane-spanning domains); ORAOV2; TMEM16A |
Gene ID : | 55107 |
mRNA Refseq : | NM_018043 |
Protein Refseq : | NP_060513 |
MIM : | 610108 |
UniProt ID : | Q5XXA6 |
Products Types
◆ Recombinant Protein | ||
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Ano1-6890M | Recombinant Mouse Ano1 protein, His & T7-tagged | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionDysregulation of ANO1 activity has been implicated in several diseases and conditions. For example, ANO1 overexpression has been observed in certain cancers, including gastrointestinal stromal tumors (GIST), breast cancer, and head and neck squamous cell carcinoma. In these cases, ANO1 may contribute to tumor growth, migration, and invasion. Moreover, mutations in the ANO1 gene have been associated with various disorders, including autosomal dominant craniocervical dystonia (a movement disorder) and certain forms of autosomal recessive gnathodiaphyseal dysplasia (a bone disorder).
ANO1 protein has been implicated in a variety of physiological processes in different tissues. In the airways, it helps regulate mucus secretion and lung function. In the gastrointestinal tract, ANO1 is involved in fluid secretion, motility, and intestinal smooth muscle contraction. It also plays a role in sensory perception, such as taste and pain sensation. Additionally, ANO1 has been found in the reproductive system, exocrine glands, and other tissues, suggesting its involvement in multiple physiological functions.
Yes, ANO1 is a member of the ANO (Anoctamin) protein family, which consists of ten members (ANO1-10). Each member has unique tissue-specific expression patterns and physiological functions. Some other members include ANO2, which is involved in neuronal excitability, ANO6, which plays a role in phospholipid scrambling, and ANO7, which is implicated in bone homeostasis. Research on other ANO family members is ongoing to understand their roles and potential therapeutic applications.
Yes, ANO1 has gained significant interest as a therapeutic target for various diseases. In cancer research, ANO1 inhibitors are being studied for their potential to suppress tumor growth and metastasis. Modulators of ANO1 have also been investigated as potential treatments for cystic fibrosis to restore abnormal chloride transport. Additionally, ANO1-related movement disorders, such as craniocervical dystonia, are being explored for potential therapeutic interventions targeting ANO1 channel function.
Several drugs have been identified that can modulate ANO1 activity. For example, a class of compounds known as Anoctamin 1 inhibitors, such as CaCCinh-A01, T16Ainh-A01, and T16Ainh-A01 hydrochloride, have been shown to selectively block ANO1 channels. These inhibitors are being studied for their potential therapeutic applications in various diseases, including cancer and cystic fibrosis.
ANO1 expression levels and alterations have been investigated as potential diagnostic and prognostic markers in certain cancers. Increased ANO1 expression has been associated with poor prognosis in various tumors, suggesting its potential as a prognostic marker. Additionally, ANO1 immunohistochemistry has been used as a diagnostic tool to differentiate GIST from other gastrointestinal tumors. However, further studies are needed to validate its clinical utility in different disease contexts.
Customer Reviews (4)
Write a reviewThe manufacturer can offer technical support and expertise to guide researchers through the experimental process.
This guarantees that the protein is reliable, consistent, and suitable for experiments, reducing variability and enhancing the reproducibility of findings.
the manufacturer can play a vital role in supporting and optimizing the research process.
This may include providing detailed protocols, troubleshooting advice, and suggestions for experimental design.
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