Recombinant Human ANO1 Protein, Met1-Ala333, N-His tagged

• Cat.No.: ANO1-12H
• Product Overview: Purified recombinant protein of Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1, Met1-Ala333, with N-terminal His tag, expressed in E.coli.

Specification

• Description: Enables calcium activated cation channel activity; intracellular calcium activated chloride channel activity; and iodide transmembrane transporter activity. Involved in cation transport; inorganic anion transport; and positive regulation of insulin secretion involved in cellular response to glucose stimulus. Located in apical plasma membrane and nucleoplasm.
• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: Met1-Ala333
• Molecular Mass: 38.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: > 0.05 μg/μL as determined by microplate BCA method
• Storage Buffer: 50 mM Tris-HCl, pH 8.0, 8 M urea

Gene Information

• Gene Name: ANO1 anoctamin 1 [ Homo sapiens (human) ]
• Official Symbol: ANO1
• Synonyms: ANO1; anoctamin 1; DOG1; INDMS; MYMY7; TAOS2; ORAOV2; TMEM16A; anoctamin-1; Ca2+-activated Cl- channel; anoctamin 1, calcium activated chloride channel; calcium activated chloride channel; discovered on gastrointestinal stromal tumors protein 1; oral cancer overexpressed 2; transmembrane protein 16A (eight membrane-spanning domains); tumor-amplified and overexpressed sequence 2
• Gene ID: 55107
• mRNA Refseq: NM_018043
• Protein Refseq: NP_060513
• MIM: 610108
• UniProt ID: Q5XXA6

Reviews

03/07/2021

The manufacturer can offer technical support and expertise to guide researchers through the experimental process.

06/18/2020

This guarantees that the protein is reliable, consistent, and suitable for experiments, reducing variability and enhancing the reproducibility of findings.

03/09/2020

the manufacturer can play a vital role in supporting and optimizing the research process.

02/21/2016

This may include providing detailed protocols, troubleshooting advice, and suggestions for experimental design.

Q&As

What diseases or conditions are associated with ANO1 dysfunction?

Dysregulation of ANO1 activity has been implicated in several diseases and conditions. For example, ANO1 overexpression has been observed in certain cancers, including gastrointestinal stromal tumors (GIST), breast cancer, and head and neck squamous cell carcinoma. In these cases, ANO1 may contribute to tumor growth, migration, and invasion. Moreover, mutations in the ANO1 gene have been associated with various disorders, including autosomal dominant craniocervical dystonia (a movement disorder) and certain forms of autosomal recessive gnathodiaphyseal dysplasia (a bone disorder).

What are the physiological roles of ANO1 in different tissues?

ANO1 protein has been implicated in a variety of physiological processes in different tissues. In the airways, it helps regulate mucus secretion and lung function. In the gastrointestinal tract, ANO1 is involved in fluid secretion, motility, and intestinal smooth muscle contraction. It also plays a role in sensory perception, such as taste and pain sensation. Additionally, ANO1 has been found in the reproductive system, exocrine glands, and other tissues, suggesting its involvement in multiple physiological functions.

Are there any other members of the ANO protein family?

Yes, ANO1 is a member of the ANO (Anoctamin) protein family, which consists of ten members (ANO1-10). Each member has unique tissue-specific expression patterns and physiological functions. Some other members include ANO2, which is involved in neuronal excitability, ANO6, which plays a role in phospholipid scrambling, and ANO7, which is implicated in bone homeostasis. Research on other ANO family members is ongoing to understand their roles and potential therapeutic applications.

Are there any ANO1-related diseases that are being researched for potential therapies?

Yes, ANO1 has gained significant interest as a therapeutic target for various diseases. In cancer research, ANO1 inhibitors are being studied for their potential to suppress tumor growth and metastasis. Modulators of ANO1 have also been investigated as potential treatments for cystic fibrosis to restore abnormal chloride transport. Additionally, ANO1-related movement disorders, such as craniocervical dystonia, are being explored for potential therapeutic interventions targeting ANO1 channel function.

Are there any known drugs that target ANO1?

Several drugs have been identified that can modulate ANO1 activity. For example, a class of compounds known as Anoctamin 1 inhibitors, such as CaCCinh-A01, T16Ainh-A01, and T16Ainh-A01 hydrochloride, have been shown to selectively block ANO1 channels. These inhibitors are being studied for their potential therapeutic applications in various diseases, including cancer and cystic fibrosis.

Can ANO1 be used as a diagnostic or prognostic marker?

ANO1 expression levels and alterations have been investigated as potential diagnostic and prognostic markers in certain cancers. Increased ANO1 expression has been associated with poor prognosis in various tumors, suggesting its potential as a prognostic marker. Additionally, ANO1 immunohistochemistry has been used as a diagnostic tool to differentiate GIST from other gastrointestinal tumors. However, further studies are needed to validate its clinical utility in different disease contexts.