Recombinant Human ASAP1 293 Cell Lysate

• Cat.No.: ASAP1-8668HCL

Specification

• Description: Antigen standard for ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ASAP1 ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 [ Homo sapiens ]
• Official Symbol: ASAP1
• Synonyms: ASAP1; ArfGAP with SH3 domain, ankyrin repeat and PH domain 1; DDEF1, development and differentiation enhancing factor 1; arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1; centaurin; beta 4; CENTB4; KIAA1249; PAP; ZG14P; DEF-1; centaurin, beta 4; PIP2-dependent ARF1 GAP; ARF GTPase-activating protein 1; development and differentiation-enhancing factor 1; ADP-ribosylation factor-directed GTPase-activating protein 1; 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein; 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein; PAG2; AMAP1; DDEF1
• Gene ID: 50807
• mRNA Refseq: NM_001247996
• Protein Refseq: NP_001234925
• MIM: 605953
• UniProt ID: Q9ULH1
• Chromosome Location: 8q24.1-q24.2
• Pathway: Arf1 pathway, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Signaling events mediated by focal adhesion kinase, organism-specific biosystem
• Function: ARF GTPase activator activity; SH3 domain binding; cytoskeletal adaptor activity; metal ion binding; protein binding; zinc ion binding

Reviews

12/25/2020

I have found the ASAP1 Protein to be highly stable, enabling long-term experiments without compromising its performance.

05/08/2019

One aspect that sets the ASAP1 Protein apart is the excellent technical support provided by the manufacturer.

01/09/2016

The ASAP1 Protein is an exceptional product that fully meets my experimental needs.

Q&As

How does ASAP1 participate in actin cytoskeleton remodeling?

ASAP1 binds to actin-binding proteins and actin regulatory proteins, enabling it to regulate actin dynamics and cytoskeleton rearrangements in response to cellular signaling.

What cellular processes are regulated by ASAP1's Arf-GAP activity?

ASAP1's Arf-GAP activity influences processes such as endocytosis, exocytosis, membrane recycling, and protein trafficking.

Is ASAP1 expression altered in cancer?

Yes, ASAP1 expression levels can be dysregulated in certain cancer types, leading to increased invasiveness and poor prognosis.

Does ASAP1 play a role in cancer development or progression?

Yes, ASAP1 has been implicated in cancer progression, promoting cell migration, invasion, and metastasis.

Where is ASAP1 primarily localized within cells?

ASAP1 is found predominantly at the plasma membrane, where it participates in various cellular activities.

What other proteins interact with ASAP1?

ASAP1 interacts with various proteins, including members of the Arf family, components of the endocytic machinery, regulators of actin dynamics, and other signal transduction molecules.

Are there any mutations or genetic variations associated with ASAP1?

Mutations or genetic variations in ASAP1 have not been extensively reported, but further research is required to explore their potential associations.