Recombinant Human ATP4A 293 Cell Lysate

• Cat.No.: ATP4A-8608HCL

Specification

• Description: Antigen standard for ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ATP4A ATPase, H+/K+ exchanging, alpha polypeptide [ Homo sapiens ]
• Official Symbol: ATP4A
• Synonyms: ATP6A
• Gene ID: 495
• mRNA Refseq: NM_000704.2
• Protein Refseq: NP_000695.2
• MIM: 137216
• UniProt ID: P20648
• Chromosome Location: 19q13.1
• Pathway: Collecting duct acid secretion, organism-specific biosystem;Collecting duct acid secretion, conserved biosystem;Gastric acid secretion, organism-specific biosystem;Gastric acid secretion, conserved biosystem;Ion channel transport, organism-specific biosystem;Ion transport by P-type ATPases, organism-specific biosystem;Oxidative phosphorylation, organism-specific biosystem
• Function: ATP binding;hydrogen:potassium-exchanging ATPase activity;magnesium ion binding;protein heterodimerization activity

Reviews

03/01/2022

After using Atp4a, the background of the Western blot was very clean with no nonspecific signal.

03/22/2020

I appreciate the stability of Atp4a, which can be kept at room temperature for several days without deterioration.

07/04/2019

By using Atp4a, the precision of my experiments was significantly improved and the results were more reliable.

Q&As

Is the polymorphism of Atp4a gene associated with abnormal gastric acid?

Yes, polymorphic variations in the Atp4a gene may be associated with the susceptibility and pathogenesis of gastric acid abnormalities, such as hyperacidity or hypoacidity.

Does Atp4a interact with proteins?

Atp4a interacts with other proteins in the Parietal cells of the gastric mucosa, such as parietal cell-associated proteins, to jointly regulate gastric acid production and secretion.

What is the mutation mechanism of Atp4a?

Mutations in Atp4a may lead to abnormal changes in its intracellular localization and function, and then affect the normal secretion of gastric acid.

How does Atp4a function under estrogen regulation?

Atp4a may be regulated by estrogen and regulate gastric acid secretion by affecting Atp4a expression and activity.

Which is the regulatory mechanism of Atp4a in gastric acid secretion?

Regulates gastric acid secretion through ATP binding and ion pump transport, and the specific mechanism involves the regulation of multiple signaling pathways and hormones.

What are the structural features of Atp4a?

Atp4a is a plasma wall protein containing several membrane channels and ATP-binding sites that regulate gastric acid secretion.