Recombinant Human ATP4A protein, His-tagged

• Cat.No.: ATP4A-19H
• Product Overview: Recombinant Human ATP4A(Leu798~Gly896) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Molecular Mass: 12.7kDa
• AA Sequence: MGHHHHHHSG SEF-LTPYLIY ITVSVPLPLG CITILFIELC TDIFPSVSLA YEKAESDIMH LRPRNPKRDR LVNEPLAAYS YFQIGAIQSF AGFTDYFTAM AQEGWFPLLC VG
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: ATP4A ATPase, H+/K+ exchanging, alpha polypeptide [ Homo sapiens ]
• Official Symbol: ATP4A
• Synonyms: ATP6A
• Gene ID: 495
• mRNA Refseq: NM_000704.2
• Protein Refseq: NP_000695
• MIM: 137216
• UniProt ID: P20648
• Chromosome Location: 19q13.1
• Pathway: Collecting duct acid secretion, organism-specific biosystem;Collecting duct acid secretion, conserved biosystem;Gastric acid secretion, organism-specific biosystem;Gastric acid secretion, conserved biosystem;Ion channel transport, organism-specific biosystem;Ion transport by P-type ATPases, organism-specific biosystem;Oxidative phosphorylation, organism-specific biosystem
• Function: ATP binding;hydrogen:potassium-exchanging ATPase activity;magnesium ion binding;protein heterodimerization activity

Reviews

03/01/2022

After using Atp4a, the background of the Western blot was very clean with no nonspecific signal.

03/22/2020

I appreciate the stability of Atp4a, which can be kept at room temperature for several days without deterioration.

07/04/2019

By using Atp4a, the precision of my experiments was significantly improved and the results were more reliable.

Q&As

Is the polymorphism of Atp4a gene associated with abnormal gastric acid?

Yes, polymorphic variations in the Atp4a gene may be associated with the susceptibility and pathogenesis of gastric acid abnormalities, such as hyperacidity or hypoacidity.

Does Atp4a interact with proteins?

Atp4a interacts with other proteins in the Parietal cells of the gastric mucosa, such as parietal cell-associated proteins, to jointly regulate gastric acid production and secretion.

What is the mutation mechanism of Atp4a?

Mutations in Atp4a may lead to abnormal changes in its intracellular localization and function, and then affect the normal secretion of gastric acid.

How does Atp4a function under estrogen regulation?

Atp4a may be regulated by estrogen and regulate gastric acid secretion by affecting Atp4a expression and activity.

Which is the regulatory mechanism of Atp4a in gastric acid secretion?

Regulates gastric acid secretion through ATP binding and ion pump transport, and the specific mechanism involves the regulation of multiple signaling pathways and hormones.

What are the structural features of Atp4a?

Atp4a is a plasma wall protein containing several membrane channels and ATP-binding sites that regulate gastric acid secretion.