Recombinant Human ATP4a, His-tagged
Cat.No. : | ATP4a-3765H |
Product Overview : | ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) |
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Description : | The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase. |
Source : | E. Coli |
Species : | Human |
Tag : | His |
Form : | Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl. |
Molecular Mass : | 12.7kDa |
Protein length : | 1035 |
Endotoxin : | <1.0eu per 1ug (determined by the lal</1.0eu |
Purity : | >95% |
Applications : | SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP) |
Notes : | Small volumes of ATP4a recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition. |
Reconstitution : | Reconstitute in sterile PBS, pH7.2-pH7.4. |
Warning : | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Gene Name : | ATP4A ATPase, H+/K+ exchanging, alpha polypeptide [ Homo sapiens ] |
Official Symbol : | ATP4a |
Synonyms : | ATP6A |
Gene ID : | 495 |
mRNA Refseq : | NM_000704.2 |
Protein Refseq : | NP_000695.2 |
MIM : | 137216 |
UniProt ID : | P20648 |
Chromosome Location : | 19q13.1 |
Pathway : | Collecting duct acid secretion, organism-specific biosystem;Collecting duct acid secretion, conserved biosystem;Gastric acid secretion, organism-specific biosystem;Gastric acid secretion, conserved biosystem;Ion channel transport, organism-specific biosys |
Function : | ATP binding;hydrogen:potassium-exchanging ATPase activity;magnesium ion binding;protein heterodimerization activity; |
Products Types
◆ Recombinant Protein | ||
Atp4a-466M | Recombinant Mouse Atp4a Protein, His/GST-tagged | +Inquiry |
ATP4A-465H | Recombinant Human ATP4A Protein, His-tagged | +Inquiry |
Atp4a-20M | Recombinant Mouse Atp4a protein, His/GST-tagged | +Inquiry |
ATP4A-975H | Recombinant Human ATP4A protein | +Inquiry |
ATP4A-19H | Recombinant Human ATP4A protein, His-tagged | +Inquiry |
◆ Lysates | ||
ATP4A-8608HCL | Recombinant Human ATP4A 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionYes, polymorphic variations in the Atp4a gene may be associated with the susceptibility and pathogenesis of gastric acid abnormalities, such as hyperacidity or hypoacidity.
Atp4a interacts with other proteins in the Parietal cells of the gastric mucosa, such as parietal cell-associated proteins, to jointly regulate gastric acid production and secretion.
Mutations in Atp4a may lead to abnormal changes in its intracellular localization and function, and then affect the normal secretion of gastric acid.
Atp4a may be regulated by estrogen and regulate gastric acid secretion by affecting Atp4a expression and activity.
Regulates gastric acid secretion through ATP binding and ion pump transport, and the specific mechanism involves the regulation of multiple signaling pathways and hormones.
Atp4a is a plasma wall protein containing several membrane channels and ATP-binding sites that regulate gastric acid secretion.
Customer Reviews (3)
Write a reviewAfter using Atp4a, the background of the Western blot was very clean with no nonspecific signal.
I appreciate the stability of Atp4a, which can be kept at room temperature for several days without deterioration.
By using Atp4a, the precision of my experiments was significantly improved and the results were more reliable.
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