Recombinant Human ATP5B 293 Cell Lysate

• Cat.No.: ATP5B-8605HCL

Specification

• Description: Antigen standard for ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ATP5B ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide [ Homo sapiens ]
• Official Symbol: ATP5B
• Synonyms: ATP5B; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; ATPSB; ATP synthase subunit beta, mitochondrial; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthetase, beta subunit; ATPMB; MGC5231
• Gene ID: 506
• mRNA Refseq: NM_001686
• Protein Refseq: NP_001677
• MIM: 102910
• UniProt ID: P06576
• Chromosome Location: 12p13.3
• Pathway: Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Electron Transport Chain, organism-specific biosystem; F-type ATPase, eukaryotes, organism-specific biosystem; Formation of ATP by chemiosmotic coupling, organism-specific biosystem; Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem
• Function: ATP binding; contributes_to ATPase activity; MHC class I protein binding; calcium ion binding; eukaryotic cell surface binding; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrogen-exporting ATPase activity, phosphorylative mechanism; hydrolase activity; hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances; lipoprotein particle receptor activity; nucleotide binding; protein binding; proton-transporting ATPase activity, rotational mechanism; transmembrane transporter activity; transporter activity

Reviews

05/22/2020

The outstanding quality of proteins lies in their strong biological activity and their high purity during production, which is really rare.

04/25/2020

ATP5B has a very short half-life and shows a very high clearance rate, which makes its biological activity more stable.

04/07/2019

Because the production process is efficient and environmentally friendly, recombinant proteins can be produced on a large scale without negative environmental impacts.

Q&As

What is the frequency of ATP5B mutations?

ATP5B gene mutation frequency may be different in different diseases and populations, which needs further study.

How about the link between ATP5B protein and neurological diseases?

It plays an important role in the nervous system and is associated with certain nervous system diseases such as Parkinson's disease and cerebral ischemia.

How are ATP5B mutations associated with disease?

This gene mutations may lead to abnormal protein structure or function, which in turn affects the physiological function of the protein and is associated with specific diseases.

What are the regulatory mechanisms of ATP5B?

ATP5B expression and function are affected by a variety of regulatory mechanisms, including regulation of transcription factors and epigenetic modifications.

How to evaluate the effect of ATP5B gene mutation on disease?

Assessment of the effect of ATP5B mutations on disease often requires a comprehensive analysis of gene sequencing, protein function studies, and clinical and epidemiological studies.

In which tissues or organs is ATP5B highly expressed?

It is highly expressed in multiple tissues and organs, especially metabolically active tissues such as heart, liver, and brain.