Recombinant Mouse Atp5b Protein, MYC/DDK-tagged

• Cat.No.: Atp5b-669M
• Product Overview: Purified recombinant protein of full-length mouse ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit (Atp5b), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 56.3 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Atp5b ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit [ Mus musculus (house mouse) ]
• Official Symbol: Atp5b
• Synonyms: Atp5b; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; Atp5f1b; ATP synthase subunit beta, mitochondrial; ATP synthase F1 subunit beta; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; EC 7.1.2.2
• Gene ID: 11947
• mRNA Refseq: NM_016774
• Protein Refseq: NP_058054
• UniProt ID: P56480

Reviews

05/22/2020

The outstanding quality of proteins lies in their strong biological activity and their high purity during production, which is really rare.

04/25/2020

ATP5B has a very short half-life and shows a very high clearance rate, which makes its biological activity more stable.

04/07/2019

Because the production process is efficient and environmentally friendly, recombinant proteins can be produced on a large scale without negative environmental impacts.

Q&As

What is the frequency of ATP5B mutations?

ATP5B gene mutation frequency may be different in different diseases and populations, which needs further study.

How about the link between ATP5B protein and neurological diseases?

It plays an important role in the nervous system and is associated with certain nervous system diseases such as Parkinson's disease and cerebral ischemia.

How are ATP5B mutations associated with disease?

This gene mutations may lead to abnormal protein structure or function, which in turn affects the physiological function of the protein and is associated with specific diseases.

What are the regulatory mechanisms of ATP5B?

ATP5B expression and function are affected by a variety of regulatory mechanisms, including regulation of transcription factors and epigenetic modifications.

How to evaluate the effect of ATP5B gene mutation on disease?

Assessment of the effect of ATP5B mutations on disease often requires a comprehensive analysis of gene sequencing, protein function studies, and clinical and epidemiological studies.

In which tissues or organs is ATP5B highly expressed?

It is highly expressed in multiple tissues and organs, especially metabolically active tissues such as heart, liver, and brain.