Recombinant Mouse ATP5B Protein (47-529 aa), His-SUMO-tagged

• Cat.No.: ATP5B-346M
• Product Overview: Recombinant Mouse ATP5B Protein (47-529 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Full Length of Mature Protein.

Specification

• Description: Mitochondrial mbrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the mbrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extrambraneous catalytic core, and F0 - containing the mbrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
• Source: E. coli
• Species: Mouse
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 67.7 kDa
• Protein length: 47-529 aa
• AA Sequence: AAQASAAPKAGTATGRIVAVIGAVV DVQFDEGLPPILNALEVQGRDSRLV LEVAQHLGESTVRTIAMDGTEGLVR GQKVLDSGAPIKIPVGPETLGRIMN VIGEPIDERGPIKTKQFAPIHAEAP EFIEMSVEQEILVTGIKVVDLLAPY AKGGKIGLFGGAGVGKTVLIMELIN NVAKAHGGYSVFAGVGERTREGNDL YHEMIESGVINLKDATSKVALVYGQ MNEPPGARARVALTGLTVAEYFRDQ EGQDVLLFIDNIFRFTQAGSEVSAL LGRIPSAVGYQPTLATDMGTMQERI TTTKKGSITSVQAIYVPADDLTDPA PATTFAHLDATTVLSRAIAELGIYP AVDPLDSTSRIMDPNIVGNEHYDVA RGVQKILQDYKSLQDIIAILGMDEL SEEDKLTVSRARKIQRFLSQPFQVA EVFTGHMGKLVPLKETIKGFQQILA GEYDHLPEQAFYMVGPIEEAVAKAD KLAEEHGS
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: Atp5b ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit [ Mus musculus ]
• Official Symbol: ATP5B
• Synonyms: ATP5B; ATP synthase subunit beta, mitochondrial
• Gene ID: 11947
• mRNA Refseq: NM_016774
• Protein Refseq: NP_058054
• UniProt ID: P56480

Reviews

05/22/2020

The outstanding quality of proteins lies in their strong biological activity and their high purity during production, which is really rare.

04/25/2020

ATP5B has a very short half-life and shows a very high clearance rate, which makes its biological activity more stable.

04/07/2019

Because the production process is efficient and environmentally friendly, recombinant proteins can be produced on a large scale without negative environmental impacts.

Q&As

What is the frequency of ATP5B mutations?

ATP5B gene mutation frequency may be different in different diseases and populations, which needs further study.

How about the link between ATP5B protein and neurological diseases?

It plays an important role in the nervous system and is associated with certain nervous system diseases such as Parkinson's disease and cerebral ischemia.

How are ATP5B mutations associated with disease?

This gene mutations may lead to abnormal protein structure or function, which in turn affects the physiological function of the protein and is associated with specific diseases.

What are the regulatory mechanisms of ATP5B?

ATP5B expression and function are affected by a variety of regulatory mechanisms, including regulation of transcription factors and epigenetic modifications.

How to evaluate the effect of ATP5B gene mutation on disease?

Assessment of the effect of ATP5B mutations on disease often requires a comprehensive analysis of gene sequencing, protein function studies, and clinical and epidemiological studies.

In which tissues or organs is ATP5B highly expressed?

It is highly expressed in multiple tissues and organs, especially metabolically active tissues such as heart, liver, and brain.