Recombinant Human DEFB103A protein, His-tagged

• Cat.No.: DEFB103A-85H
• Product Overview: Recombinant Human DEFB103A (Gly 23-Lys 67), fussed with His tag at N-terminal, was expressed in E. coli.

Specification

• Description: Beta-defensin 3 is a member of the defensin family. Defensin family is comprised by microbicidal and cytotoxic peptides made by neutrophils. Members of the beta-defensin 3 family are highly similar in protein sequence. Beta-defensin 3 shows antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Beta-defensin 3 is abundantly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. It is also expressed in salivary gland, bone marrow, colon, stomach, polyp and larynx. However, in small intestine, it cannot be detected. Defensin has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. Beta-defensin 3 kills multiresistant S.aureus and vancomycin-resistent E.faecium. It has no significant hemolytic activity.
• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 50mM Tris, 0.3% Ttiton X-100, 0.3% SKL, pH 8.51. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
• Molecular Mass: The recombinant human DEFB103A consists of 61 amino acids and predicts a molecular mass of 7.3 KDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.
• Protein length: Gly 23-Lys 67
• Purity: > 99 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C
• Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: DEFB103A defensin, beta 103A [ Homo sapiens ]
• Official Symbol: DEFB103A
• Synonyms: DEFB103A; defensin, beta 103A; DEFB3, DEFB103, defensin, beta 3; beta-defensin 103; DEFB 3; HBD 3; HBD3; HBP 3; HBP3; beta-defensin 3; defensin, beta 3; defensin, beta 103; defensin-like protein; BD-3; DEFB3; HBP-3; hBD-3; DEFB-3; DEFB103
• Gene ID: 414325
• mRNA Refseq: NM_001081551
• Protein Refseq: NP_001075020
• UniProt ID: P81534
• Chromosome Location: 8p23.1
• Pathway: Beta defensins, organism-specific biosystem; Defensins, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem

Reviews

10/11/2022

This unique capability to selectively bind to exposed PS simplifies the identification and quantification of different cellular populations, reducing experimental errors and enhancing accuracy.

06/23/2018

They can provide technical assistance, ensuring optimal utilization of the product.

10/28/2017

This may include providing detailed protocols, troubleshooting guidance, and access to knowledgeable experts who can answer any inquiries or concerns.

Q&As

What specific clinical conditions are associated with DEFB103A deficiency?

DEFB103A deficiency has been linked to an increased susceptibility to various infections, particularly those affecting the skin and mucosal surfaces.

Are there any side effects or concerns associated with the therapeutic use of DEFB103A?

While research is ongoing, potential side effects and concerns related to the therapeutic use of DEFB103A or its analogs need to be thoroughly investigated, including any possible immunogenicity.

How does DEFB103A interact with the human microbiome?

DEFB103A plays a role in maintaining a balanced microbiome by exerting selective antimicrobial activity. Understanding this interaction is crucial for potential applications in microbiome-related disorders.

In what ways does DEFB103A play a role in inflammatory diseases?

DEFB103A is implicated in modulating inflammation. It can influence the immune response and may have a role in inflammatory conditions such as psoriasis and inflammatory bowel disease.

How does DEFB103A contribute to the immune system?

DEFB103A plays a crucial role in the innate immune system by acting as an antimicrobial peptide. It helps defend the body against a wide range of pathogens, including bacteria, viruses, and fungi.