Recombinant Human DNMT3A, His-tagged

• Cat.No.: DNMT3A-390H
• Product Overview: The enzymes are His-tagged and overexpressed in Baculovirus. Lysates are produced by sonication in the presence of protease inhibitors and the proteins affinity purified over nickel-agarose columns (eluted with imidazole buffer).

Specification

• Description: DNA methylation at CpG sites is an essential feature of gene regulation in higher eukarya. Methylation of cytosine residues is correlated with silenced chromatin and there is strong evidence that inappropriate methylation can disrupt normal growth control circuits. The process is mediated by enzymes called DNA methyltransferases (DNMTs) and a number of different DNMT isoforms have been identified, cloned and characterized. DNMT isoforms fall into two broad groupings: 1) Maintenance activities which preferentially act on hemi-methylated DNA, and 2) De novo activities which initiate methylation on a unmethylated DNA targets. DNMT1 is a maintenance enzyme that most likely targets replicative or nascent DNA. DNMT3 type isoforms appear to be de novo methyltransferases. Other DNMTs are now known to be active but have not been classified (DNMT2) while still other isoforms appear to possess key motifs of DNMT but lack sequences necessary for catalytic activity (DNMT3L).
• Source: Baculovirus
• Species: Human
• Tag: His
• Storage: The enzymes are shipped on Dry Ice. Detailed temperature stabilization time profiles have not been performed; however, store at -20oC to -70oC and avoid repeat freeze thawing cycles (it is best to aliquot the enzyme on first thaw). The enzymes are stable for 6 months from date of shipping under these conditions.

Gene Information

• Gene Name: DNMT3A DNA (cytosine-5-)-methyltransferase 3 alpha [ Homo sapiens ]
• Official Symbol: DNMT3A
• Synonyms: DNMT3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA (cytosine-5)-methyltransferase 3A; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2; DNMT3A2; M.HsaIIIA
• Gene ID: 1788
• mRNA Refseq: NM_022552
• Protein Refseq: NP_072046
• MIM: 602769
• UniProt ID: Q9Y6K1
• Chromosome Location: 2p23
• Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Methionine degradation, organism-specific biosystem; Methionine degradation, conserved biosystem; One Carbon Metabolism, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem
• Function: DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; chromatin binding; metal ion binding; methyltransferase activity; protein binding; transferase activity; unmethylated CpG binding

Reviews

11/17/2022

Its exceptional characteristics, coupled with the manufacturer's commitment to customer satisfaction, ensure that I can pursue my scientific investigations with confidence and success.

09/07/2022

Its consistent performance and reproducibility have been praised by scientists who have employed it in their studies, with many reporting outstanding results and a high level of satisfaction.

08/20/2017

This level of support becomes invaluable, allowing researchers to focus on their scientific objectives while receiving expert guidance alongside.

Q&As

Can DNMT3A protein be used as a biomarker for cancer diagnosis?

DNMT3A mutations can serve as biomarkers for certain cancers, aiding in the diagnosis and classification of hematologic malignancies.

How does DNMT3A impact normal cellular function?

DNMT3A is essential for normal DNA methylation patterns during development and differentiation, influencing gene expression and cellular function.

Can DNMT3A-targeted therapies have off-target effects?

Like many targeted therapies, DNMT3A-targeted treatments may have off-target effects, and their specificity is an area of active research.

Are there diagnostic tests for detecting DNMT3A mutations?

Yes, molecular diagnostic tests, such as DNA sequencing, can identify DNMT3A mutations in cancer cells.

How does DNMT3A contribute to epigenetic changes in cancer cells?

DNMT3A mutations lead to abnormal DNA methylation patterns, contributing to altered gene expression and promoting cancer development.