Recombinant Human DNMT3A, His-tagged

• Cat.No.: DNMT3A-20H
• Product Overview: Recombinant Human DNMT3A protein(NP_072046.2)(680-902aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 29.9kDa
• Protein length: 680-902aa
• AA Sequence: KIMYVGDVRSVTQKHIQEWGPFDLV IGGSPCNDLSIVNPARKGLYEGTGR LFFEFYRLLHDARPKEGDDRPFFWL FENVVAMGVSDKRDISRFLESNPVM IDAKEVSAAHRARYFWGNLPGMNRP LASTVNDKLELQECLEHGRIAKFSK VRTITTRSNSIKQGKDQHFPVFMNE KEDILWCTEMERVFGFPVHYTDVSN MSRLARQRLLGRSWSVPVIRHLF
• Purity: Greater than 90% as determined by SDS-PAGE.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: DNMT3A DNA (cytosine-5-)-methyltransferase 3 alpha [ Homo sapiens ]
• Official Symbol: DNMT3A
• Synonyms: DNMT3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA (cytosine-5)-methyltransferase 3A; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2; DNMT3A2; M.HsaIIIA
• Gene ID: 1788
• mRNA Refseq: NM_022552.4
• Protein Refseq: NP_072046.2
• MIM: 602769
• UniProt ID: Q9Y6K1

Reviews

11/17/2022

Its exceptional characteristics, coupled with the manufacturer's commitment to customer satisfaction, ensure that I can pursue my scientific investigations with confidence and success.

09/07/2022

Its consistent performance and reproducibility have been praised by scientists who have employed it in their studies, with many reporting outstanding results and a high level of satisfaction.

08/20/2017

This level of support becomes invaluable, allowing researchers to focus on their scientific objectives while receiving expert guidance alongside.

Q&As

Can DNMT3A protein be used as a biomarker for cancer diagnosis?

DNMT3A mutations can serve as biomarkers for certain cancers, aiding in the diagnosis and classification of hematologic malignancies.

How does DNMT3A impact normal cellular function?

DNMT3A is essential for normal DNA methylation patterns during development and differentiation, influencing gene expression and cellular function.

Can DNMT3A-targeted therapies have off-target effects?

Like many targeted therapies, DNMT3A-targeted treatments may have off-target effects, and their specificity is an area of active research.

Are there diagnostic tests for detecting DNMT3A mutations?

Yes, molecular diagnostic tests, such as DNA sequencing, can identify DNMT3A mutations in cancer cells.

How does DNMT3A contribute to epigenetic changes in cancer cells?

DNMT3A mutations lead to abnormal DNA methylation patterns, contributing to altered gene expression and promoting cancer development.