Recombinant Human SUMF1 293 Cell Lysate

• Cat.No.: SUMF1-1346HCL

Specification

• Description: Antigen standard for sulfatase modifying factor 1 (SUMF1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SUMF1 sulfatase modifying factor 1 [ Homo sapiens ]
• Official Symbol: SUMF1
• Synonyms: SUMF1; sulfatase modifying factor 1; sulfatase-modifying factor 1; FGE; UNQ3037; FGly-generating enzyme; C-alpha-formylglycine-generating enzyme 1; AAPA3037; MGC131853; MGC150436
• Gene ID: 285362
• mRNA Refseq: NM_001164674
• Protein Refseq: NP_001158146
• MIM: 607939
• UniProt ID: Q8NBK3
• Chromosome Location: 3p26.1
• Pathway: Lysosome, organism-specific biosystem; Lysosome, conserved biosystem

Reviews

02/10/2022

Expert protein engineering support, an invaluable resource for our project.

06/14/2019

Consistent western blot results, a lab necessity for our research.

05/17/2019

Great protein crystallization service, vital for our structural biology work.

Q&As

What is the impact of SUMF1 on lysosomal function and storage diseases?

SUMF1 plays a role in lysosomal function, with implications for lysosomal storage diseases.

How does SUMF1 influence bone development and skeletal disorders?

SUMF1 influences bone growth and development, potentially affecting skeletal health and disorders.

What role does SUMF1 play in the degradation of glycosaminoglycans and mucopolysaccharides?

SUMF1 aids in the degradation of glycosaminoglycans, crucial for the breakdown of these complex molecules.

What are the mechanisms by which SUMF1 modulates cellular detoxification processes?

SUMF1 contributes to cellular detoxification, aiding in the breakdown of potentially harmful substances.

How do alterations in SUMF1 expression or function affect neurological development and function?

Variations in SUMF1 activity can impact neurological development and function, influencing brain health and disease.

How does SUMF1 deficiency contribute to the development of multiple sulfatase deficiency (MSD) and related symptoms?

SUMF1 deficiency leads to multiple sulfatase deficiency, disrupting normal metabolism and causing various symptoms.

How does SUMF1 (Sulfatase Modifying Factor 1) activate sulfatase enzymes involved in cellular metabolism?

SUMF1 is essential for the activation of sulfatase enzymes, impacting their role in cellular metabolism.