Recombinant Human SYT3 293 Cell Lysate

• Cat.No.: SYT3-1305HCL

Specification

• Description: Antigen standard for synaptotagmin III (SYT3), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SYT3 synaptotagmin III [ Homo sapiens ]
• Official Symbol: SYT3
• Synonyms: SytIII
• Gene ID: 84258
• mRNA Refseq: NM_032298.2
• Protein Refseq: NP_115674.1
• MIM: 600327
• UniProt ID: Q9BQG1
• Chromosome Location: 19q13.33

Reviews

07/18/2021

Its purity and consistency enable accurate and precise data analysis, ensuring the integrity of my research outcomes.

02/09/2017

the SYT3 protein's exceptional quality, reliability, and the manufacturer's unparalleled technical support make it a prime choice for researchers seeking to delve into the intricacies of angiogenesis, vascular development, and related fields.

07/10/2016

As a researcher utilizing the SYT3 protein in my trials, I have been highly impressed with its numerous advantages that significantly contribute to the success of my experimental endeavors.

Q&As

How does SYT3 contribute to the regulation of calcium signaling in neurons?

SYT3's calcium-binding properties are essential for its role in synaptic vesicle fusion, contributing to the finely tuned calcium signaling necessary for proper neuronal function.

How can SYT3 protein be utilized in diagnostics?

SYT3 levels or mutations might serve as biomarkers for neurological disorders, aiding in the development of diagnostic tools to identify and understand these conditions.

Are there any genetic mutations associated with SYT3 and neurological disorders?

Research suggests that genetic variations in SYT3 may be linked to certain neurological conditions, providing insights into potential genetic markers for susceptibility.

Can SYT3 be a target for drug development?

Explore the potential of SYT3 as a target for drug development, considering its role in neurotransmitter release and synaptic function.

Are there any ongoing clinical trials targeting SYT3 for therapeutic interventions?

Investigate current research and clinical trials focused on modulating SYT3 function as a potential therapeutic strategy for neurological disorders.