Recombinant Human TCF7L2 293 Cell Lysate

• Cat.No.: TCF7L2-1177HCL

Specification

• Description: Antigen standard for transcription factor 7-like 2 (T-cell specific, HMG-box) (TCF7L2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: TCF7L2 transcription factor 7-like 2 (T-cell specific, HMG-box) [ Homo sapiens ]
• Official Symbol: TCF7L2
• Synonyms: TCF7L2; transcription factor 7-like 2 (T-cell specific, HMG-box); TCF4; transcription factor 7-like 2; TCF 4; hTCF-4; T-cell factor 4; T-cell factor-4 variant A; T-cell factor-4 variant B; T-cell factor-4 variant C; T-cell factor-4 variant D; T-cell factor-4 variant E; T-cell factor-4 variant F; T-cell factor-4 variant G; T-cell factor-4 variant H; T-cell factor-4 variant I; T-cell factor-4 variant J; T-cell factor-4 variant K; T-cell factor-4 variant L; T-cell factor-4 variant M; T-cell factor-4 variant X2; HMG box transcription factor 4; T-cell-specific transcription factor 4; TCF-4
• Gene ID: 6934
• mRNA Refseq: NM_001146274
• Protein Refseq: NP_001139746
• MIM: 602228
• UniProt ID: Q9NQB0
• Chromosome Location: 10q25.3
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Basal cell carcinoma, organism-specific biosystem

Reviews

02/28/2021

Exceptional expertise, elevates our research outcomes.

10/27/2018

Exemplary service, vital for our studies.

07/17/2017

Timely and accurate results, invaluable for research.

Q&As

How does TCF7L2 interact with other transcription factors and signaling molecules?

TCF7L2 works in concert with other transcription factors and signaling molecules to regulate gene expression.

How do genetic variations in the TCF7L2 gene affect its function and contribute to disease susceptibility?

Genetic variations in TCF7L2 can significantly impact its function, increasing susceptibility to diabetes and other conditions.

What are the implications of altered TCF7L2 activity in cancer biology?

Altered TCF7L2 activity is associated with various cancers, affecting tumor growth and metastasis.

What is TCF7L2's function in Wnt signaling pathway and its impact on cell proliferation and differentiation?

In the Wnt signaling pathway, TCF7L2 regulates genes involved in cell growth, proliferation, and differentiation.

What potential therapeutic strategies could target TCF7L2 in various diseases, including diabetes and cancer?

Targeting TCF7L2 in therapies could offer new approaches in managing diabetes and certain cancers.

What is the primary role of TCF7L2 in gene regulation and cellular signaling?

TCF7L2 is a transcription factor that regulates gene expression, playing a crucial role in various cellular processes.

How does TCF7L2 influence the development and progression of diabetes, particularly type 2 diabetes?

It is strongly linked to type 2 diabetes risk, influencing insulin secretion and glucose metabolism.