Recombinant Human TDP1 293 Cell Lysate
Cat.No. : | TDP1-1155HCL |
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Description : | Antigen standard for tyrosyl-DNA phosphodiesterase 1 (TDP1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | TDP1 tyrosyl-DNA phosphodiesterase 1 [ Homo sapiens ] |
Official Symbol : | TDP1 |
Synonyms : | TDP1; tyrosyl-DNA phosphodiesterase 1; FLJ11090; SCAN1; tyr-DNA phosphodiesterase 1; MGC104252; |
Gene ID : | 55775 |
mRNA Refseq : | NM_001008744 |
Protein Refseq : | NP_001008744 |
MIM : | 607198 |
UniProt ID : | Q9NUW8 |
Chromosome Location : | 14q32.11 |
Pathway : | DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Nonhomologous End-joining (NHEJ), organism-specific biosystem; Processing of DNA ends prior to end rejoining, organism-specific biosystem; |
Products Types
◆ Recombinant Protein | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (4)
Ask a questionInhibition of TDP1 expression induces apoptosis in gastric cancer cells.
TDP1 has been closely associated with the study of diseases such as gastric cancer and lung cancer.
Lung adenocarcinoma tissues expressed higher levels of TDP1 compared to paraneoplastic and normal tissues.
TDP1 is involved in DNA damage repair and plays a driving role in tumorigenesis and development.
Customer Reviews (3)
Write a reviewVery professional customer service, fully understanding the needs of the customer and giving professional advice.
The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.
Fast and timely shipment helped me to get satisfactory experimental results in short time.
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