Recombinant Mouse Apln protein, His & GST-tagged

• Cat.No.: Apln-8263M
• Product Overview: Recombinant Mouse Apln aa. (Val23~Phe77 (Accession # Q9R0R4)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes the neuropeptide Apelin, an endogenous ligand of the G protein-coupled receptor APJ. This gene is highly expressed in the central nervous system and peripheral tissues and the encoded preproprotein undergoes proteolytic processing to generate mature peptides of different sizes that are secreted into the plasma. These peptides play important roles in a broad range of physiological processes such as cardiac contractility, blood pressure, blood vessel growth, appetite and drink behavior, and pituitary hormone secretion. Mice lacking the encoded protein develop progressive heart failure and exhibit a shorter bleeding time, prothrombotic profile and increased bone mass.
• Source: E. coli
• Species: Mouse
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 38.6kDa.
• Protein length: Val23~Phe77 (Accession # Q9R0R4)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: Apln apelin [ Mus musculus (house mouse) ]
• Official Symbol: Apln
• Synonyms: Apln; apelin; Apel; 6030430G11Rik; APJ endogenous ligand; preproapelin
• Gene ID: 30878
• mRNA Refseq: NM_013912.4
• Protein Refseq: NP_038940.1
• UniProt ID: Q9R0R4

Reviews

12/16/2022

Western blot experiments using this protein can obtain clearly distinguishable bands, indicating that it has high clarity and legibility.

02/10/2022

In the course of repeated experiments, the stability of this protein has remained consistent, which provides us with great convenience.

12/12/2019

I appreciate the manufacturer's pursuit of product quality, and their product repeatability is very good, which makes me more confident in the experimental results.

Q&As

Does the study of APLN involve genetic engineering technology?

Yes, genetic engineering techniques are often used to produce APLN s.

Does APLN interact with other drugs?

There may be interactions, and it is recommended to avoid concurrent use of other drugs during use, especially those that affect the cardiovascular system.

What is the action mechanism of APLN ?

APLN promotes cardiovascular protection and anti-tumor effects by binding to APJ receptors.

In what diseases might APLN have potential applications?

APLN has potential applications in the treatment of cardiovascular diseases, tumors and metabolic diseases.

In what way can APLN be administered?

APLN can be administered intravenously or subcutaneously.

What is the dose of APLN to treat cardiovascular disease?

Dosage should be determined according to the specific situation, it is recommended to consult with a professional doctor.

Does the development of APLN involve animal experiments?

In the development process, it usually goes through a series of animal experiments to verify its effectiveness and safety.

What are the side effects of APLN ?

Common side effects include injection site pain, fever, nausea, etc., but most patients tolerate them well.

What is the protective effect of APLN on cardiovascular diseases?

APLN can promote blood vessel dilation, inhibit inflammatory response, and facilitate the survival and repair of cardiomyocytes.

Has the application of APLN in tumor therapy been clinically verified?

There are currently no large-scale clinical trials to confirm its use in cancer treatment, but preliminary research results are promising.