Recombinant Full Length Human ATP4A Protein

• Cat.No.: ATP4A-1297HF
• Product Overview: Human ATP4A full-length ORF (AAI67780.1) recombinant protein without tag.

Specification

• Description: The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]
• Source: In Vitro Cell Free System
• Species: Human
• Form: Liquid
• Molecular Mass: 113.9 kDa
• Protein Length: 1035 amino acids
• AA Sequence: MGKAENYELY SVELGPGPGG DMAAKMSKKK KAGGGGGKRK EKLENMKKEM EINDHQLSVA ELEQKYQTSA TKGLSASLAA ELLLRDGPNA LRPPRGTPEY VKFARQLAGG LQCLMWVAAA ICLIAFAIQA SEGDLTTDDN LYLAIALIAV VVVTGCFGYY QEFKSTNIIA SFKNLVPQQA TVIRDGDKFQ INADQLVVGD LVEMKGGDRV PADIRILAAQ GCKVDNSSLT GESEPQTRSP ECTHESPLET RNIAFFSTMC LEGTVQGLVV NTGDRTIIGR IASLASGVEN EKTPIAIEIE HFVDIIAGLA ILFGATFFIV AMCIGYTFLR AMVFFMAIVV AYVPEGLLAT VTVCLSLTAK RLASKNCVVK NLEAVETLGS TSVICSDKTG TLTQNRMTVS HLWFDNHIHT ADTTEDQSGQ TFDQSSETWR ALCRVLTLCN RAAFKSGQDA VPVPKRIVIG DASETALLKF SELTLGNAMG YRDRFPKVCE IPFNSTNKFQ LSIHTLEDPR DPRHLLVMKG APERVLERCS SILIKGQELP LDEQWREAFQ TAYLSLGGLG ERVLGFCQLY LNEKDYPPGY AFDVEAMNFP SSGLCFAGLV SMIDPPRATV PDAVLKCRTA GIRVIMVTGD HPITAKAIAA SVGIISEGSE TVEDIAARLR VPVDQVNRKD ARACVINGMQ LKDMDPSELV EALRTHPEMV FARTSPQQKL VIVESCQRLG AIVAVTGDGV NDSPALKKAD IGVAMGIAGS DAAKNAADMI LLDDNFASIV TGVEQGRLIF DNLKKSIAYT LTKNIPELTP YLIYITVSVP LPLGCITILF IELCTDIFPS VSLAYEKAES DIMHLRPRNP KRDRLVNEPL AAYSYFQIGA IQSFAGFTDY FTAMAQEGWF PLLCVGLRAQ WEDHHLQDLQ DSYGQEWTFG QRLYQQYTCY TVFFISIEVC QIADVLIRKT RRLSAFQQGF FRNKILVIAI VFQVCIGCFL CYCPGMPNIF NFMPIRFQWW LVPLPYGILI FVYDEIRKLG VRCCPGSWWD QELYY
• Applications: Antibody Production; Functional Study; Compound Screening
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 25 mM Tris-HCl of pH8.0 containing 2% glycerol.

Gene Information

• Gene Name: ATP4A ATPase, H+/K+ exchanging, alpha polypeptide [ Homo sapiens ]
• Official Symbol: ATP4A
• Synonyms: ATP6A
• Gene ID: 495
• mRNA Refseq: NM_000704.2
• Protein Refseq: NP_000695.2
• MIM: 137216
• UniProt ID: P20648

Reviews

03/01/2022

After using Atp4a, the background of the Western blot was very clean with no nonspecific signal.

03/22/2020

I appreciate the stability of Atp4a, which can be kept at room temperature for several days without deterioration.

07/04/2019

By using Atp4a, the precision of my experiments was significantly improved and the results were more reliable.

Q&As

Is the polymorphism of Atp4a gene associated with abnormal gastric acid?

Yes, polymorphic variations in the Atp4a gene may be associated with the susceptibility and pathogenesis of gastric acid abnormalities, such as hyperacidity or hypoacidity.

Does Atp4a interact with proteins?

Atp4a interacts with other proteins in the Parietal cells of the gastric mucosa, such as parietal cell-associated proteins, to jointly regulate gastric acid production and secretion.

What is the mutation mechanism of Atp4a?

Mutations in Atp4a may lead to abnormal changes in its intracellular localization and function, and then affect the normal secretion of gastric acid.

How does Atp4a function under estrogen regulation?

Atp4a may be regulated by estrogen and regulate gastric acid secretion by affecting Atp4a expression and activity.

Which is the regulatory mechanism of Atp4a in gastric acid secretion?

Regulates gastric acid secretion through ATP binding and ion pump transport, and the specific mechanism involves the regulation of multiple signaling pathways and hormones.

What are the structural features of Atp4a?

Atp4a is a plasma wall protein containing several membrane channels and ATP-binding sites that regulate gastric acid secretion.