Recombinant Mouse Atp4a protein, His/GST-tagged

• Cat.No.: Atp4a-20M
• Product Overview: Recombinant Mouse Atp4a(Pro786~Cys1014) fused with His/GST tag at N-terminal was expressed in E. coli.

Specification

• Description: ATPase, H+/K+ exchanging, gastric, alpha polypeptide played an important role in many functions.
• Source: E. coli
• Species: Mouse
• Tag: His/GST
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Molecular Mass: 58.7kDa
• AA Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- PELTP YLIYITVSVP LPLGCITILF IELCTDIFPS VSLAYEKAES DIMHLRPRNP KRDRLVNEPL AAYSYFQIGA IQSFAGFADY FTAMAQEGWF PLLCVGLRPQ WEDHHLQDLQ DSYGQEWTFG QRLYQQYTCY TVFFISIEMC QIADVLIRKT RRLSVFQQGF FRNKILVIAI VFQVCIGCFL CYCPGMPNIF NFMPIRFQWW LVPMPFGLLI FVYDEIRKLG VRCC
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Atp4a ATPase, H+/K+ exchanging, gastric, alpha polypeptide [ Mus musculus ]
• Official Symbol: Atp4a
• Synonyms: potassium-transporting ATPase alpha chain 1; ATPase, H+/K+ exchanging, alpha polypeptide; ATPase, H+/K+ transporting, alpha polypeptide; H+/K+-ATPase alpha; H+K+-transporting alpha 1; gastric H(+)/K(+) ATPase subunit alpha; proton pump
• Gene ID: 11944
• mRNA Refseq: NM_001290627
• Protein Refseq: NP_001277556
• Chromosome Location: 7 B1; 7 19.2 cM
• Pathway: Collecting duct acid secretion, organism-specific biosystem; Gastric acid secretion, conserved biosystem; Ion transport by P-type ATPases, organism-specific biosystem
• Function: ATP binding; hydrogen:potassium-exchanging ATPase activity; magnesium ion binding

Reviews

03/01/2022

After using Atp4a, the background of the Western blot was very clean with no nonspecific signal.

03/22/2020

I appreciate the stability of Atp4a, which can be kept at room temperature for several days without deterioration.

07/04/2019

By using Atp4a, the precision of my experiments was significantly improved and the results were more reliable.

Q&As

Is the polymorphism of Atp4a gene associated with abnormal gastric acid?

Yes, polymorphic variations in the Atp4a gene may be associated with the susceptibility and pathogenesis of gastric acid abnormalities, such as hyperacidity or hypoacidity.

Does Atp4a interact with proteins?

Atp4a interacts with other proteins in the Parietal cells of the gastric mucosa, such as parietal cell-associated proteins, to jointly regulate gastric acid production and secretion.

What is the mutation mechanism of Atp4a?

Mutations in Atp4a may lead to abnormal changes in its intracellular localization and function, and then affect the normal secretion of gastric acid.

How does Atp4a function under estrogen regulation?

Atp4a may be regulated by estrogen and regulate gastric acid secretion by affecting Atp4a expression and activity.

Which is the regulatory mechanism of Atp4a in gastric acid secretion?

Regulates gastric acid secretion through ATP binding and ion pump transport, and the specific mechanism involves the regulation of multiple signaling pathways and hormones.

What are the structural features of Atp4a?

Atp4a is a plasma wall protein containing several membrane channels and ATP-binding sites that regulate gastric acid secretion.